不同治法方药治疗小鼠自身免疫性卵巢功能衰退的比较研究

目的：比较补肾法、活血法、疏肝法对小鼠自身免疫性卵巢功能衰退（premature ovarian failure，POF）的干预作用。方法：以BALB／C雌性小鼠卵巢组织制备卵巢抗原。皮下和两后脚掌多点注射免疫同种雌性小鼠建立自身免疫性卵巢功能衰退模型。六味地黄丸、少腹逐瘀颗粒及柴胡疏肝丸分别治疗3周后，酶联免疫吸附测定法检测血清雌二醇（estradiol，E2）、卵泡刺激素（follicle—stimulating hormone，FSH）、黄体生成素（luteinizing hormone，LH）、催乳素（prolactin，PRL）、睾酮（testosterone，T）以及抗卵巢抗体和抗透明带抗体。结果：模型组小鼠血清FSH、LH较正常组明显升高，E2显著下降（P〈0．05），PRL、T含量与正常组比较差异无统计学意义（P〉0．05）；模型组小鼠抗卵巢抗体阳性率为84．2％，抗透明带抗体阳性率为78．9％。与正常组相比，差异有统计学意义（P〈0．05）。六味地黄丸显著下调小鼠血清FSH、LH水平，提高E2含量（P〈0．05）；而抗卵巢抗体及抗透明带抗体仅有下降趋势，与模型组比较差异无统计学意义（P〉0．05）。少腹逐瘀颗粒组小鼠血清FSH、LH含量有下降趋势，E2含量稍有升高，但差异无统计学意义（P〉0．05）；其抗卵巢抗体和抗透明带抗体阳性率与模型组相比，差异有统计学意义（P〈0．05）。柴胡疏肝丸组小鼠抗卵巢抗体和抗透明带抗体阳性率，与模型组相比差异有统计学意义（P〈0．05）。各种治疗对血清PRL和T含量无明显影响。结论：补肾中药可以显著改善自身免疫性卵巢功能衰退小鼠生殖内分泌轴功能，活血及疏肝中药可以调整自身免疫性卵巢功能衰退小鼠体液免疫功能。

Comparative study of different therapeutic methods on autoimmune premature ovarian failure in mice

Objective： To investigate the effects of different methods of treatment, including tonifying the kidney, activating blood and soothing the liver, on mice with autoimmune premature ovarian failure （POF）. Methods： After preparing ovarian antigen with the ovarian tissue of female BALB/C mice, PQF in female BALB/C mice was induced by immunization injection of the ovarian antigen on multiple subcutaneous sites andtwo posterior soles. The mice were treated with Liuwei Dihuang Pill, Shaofu Zhuyu Granule, Chaihu Shugan Pill respectively after the third immunization. Levels of follicle-stimulating hormone （FSH）, luteinizing hormone （LH）, estradiol （E2）, prolactin （PRL）, testosterone （T）, anti-ovarian antibodies （AOAb） and anti-zona pellucida antibody （aZP） in sera were analyzed by enzyme-linked immunosorbent assay.Results： Compared with the normal group, the levels of serum FSH and LH were increased and the level of E2 was decreased in the untreated group. AOAb and aZP positive rates in the untreated group were obviously higher than those in the normal group. Liuwei Dihuang Pill could significantly reduce the levels of FSH and LH and increase the level of E2 as compared with the untreated group, but there were no significant differences in the AOAb and aZP positive rates. Shaofu Zhuyu Granule and Chaihu Shugan Pill could significantly reduce the positive rates of AOAb and aZP as compared with the untreated group, but no significant effect on the levels of FSH, LH and E2 were observed. The drugs had no effects on serum contents of PRL and T.Conclusion： Tonifying the kidney method can recover the ovarian function in POF mice mainly by regulating the hormone levels. Activating blood and soothing the liver methods can improve the humoral immune function in POF mice.