室间隔缺损血清标志物SPARC的验证研究

目的：验证富含半胱氨酸的酸性分泌蛋白（secreted protein acidic and rich in cysteine,SPARC）是否为室间隔缺损（ventricular septal defect,VSD）的潜在血清标志物。方法：收集性别、年龄、民族频数匹配的室间隔缺损、房间隔缺损（atrial septal defect,ASD）、法洛四联症（tetralogy of Fallot,TOF）患者和健康对照者血清各20例,应用Western blot和ELISA对各组血清样本中SPARC的表达水平进行检测。结果：Western blot结果表明,SPARC均在4组血清中表达,但VSD组中的相对表达水平均高于其他3组（P〈0.05）;ELISA检测结果显示VSD组患者血清中SPARC浓度为（198.03±87.26）ng/L,ASD组为（137.02±83.89）ng/L,TOF组为（140.61±79.98）ng/L,健康对照组为（91.30±55.50）ng/L,4组间比较差异有统计学意义（P〈0.05）,进一步两两比较,VSD组与其他3组差异均有统计学意义（P〈0.05）,而其他3组两两间差异均无统计学意义（P〉0.05）。结论：SPARC在VSD患者血清中表达水平增高,可能是VSD潜在的血清标志物,可为VSD的早期诊断及发病机制研究提供线索。

VERIFICATION OF SPARC AS A SERUM CANDIDATE BIOMARKER FOR VENTRICULAR SEPTAL DEFECT

Objective：To validate whether secreted protein acidic and rich in cysteine （SPARC） can be used as a serum candidate biomarker of patients with ventricular septal defect （VSD）. Methods： Serum samples were obtained from patients with VSD, atrial septal defect （ASD）, tetralogy of Fallot （TOF）, and healthy controls. Each group had 20 research objects. The four groups were frequency matched in age, gender, and nationality, respectively. The expression levels of SPARC in serum samples were detected by western blot and ELISA. Results： Western blot analysis showed that SPARC was detected in all samples of research objects. However, the relative expression level of SPARC in VSD group was higher than that in the other three groups （ P 〈0.05）. The results of ELISA indicated that the serum concentrations of SPARC in VSD, ASD, TOF and healthy group were （198.03±87.26）, （137.02±83.89）, （140.61±79.98） and （91.30± 55.50） ng/L, respectively （ P 〈0.05）. There were significant differences between VSD group and the other three groups respectively （ P 〈0.05）, but no difference among the other three groups （ P 〉0.05）. Conclusion.. Serum SPARC concentration is high in patients with VSD, which suggests that SPARC may be a serum candidate biomarker for VSD and provide clues for VSD＇s early diagnosis and explore the pathogenesis of VSD.