谷胱甘肽S-转移酶M1、T1基因多态性与慢性阻塞性肺疾病易感性关系的研究

目的　探讨谷胱甘肽S 转移酶系 (GSTs)中GSTM1、GSTT1基因多态性与慢性阻塞性肺疾病 (COPD)易感性的关系。方法　COPD患者 14 6例 ,健康吸烟对照组 10 9例。采用多重PCR方法检测GSTM1和GSTT1基因多态性 ,运用SPSS10 0统计软件进行数据处理和分析。结果　COPD组和对照组的GSTM1纯合缺失型分别为 5 8 9%和 5 2 3% ,GSTT1纯合缺失型分别为 4 5 2 %和4 5 0 % ,GSTM1和GSTT1同时纯合缺失型分别为 2 7 4 %和 19 3% ,三种基因型两组之间差异均无显著性 (P >0 0 5 )。经调整年龄、性别及吸烟指数后 ,COPD组和对照组三种基因型比例差异仍无显著性 (P >0 0 5 )。结论　未发现GSTM1、GSTT1基因多态性与COPD易感性相关 ,未发现两个基因之间有明显的协同作用。

Association of genetic polymorphism of glutathione S-transferase M1 and T1 with susceptibility to chronic obstructive pulmonary disease

Objective To investigate the association of genetic polymorphism of glutathione S-transferase M1 (GST M1) and T1 (GST T1) with susceptibility to chronic pulmonary disease (COPD).Methods The genotypes of GST M1 and GST T1 were determined by multiplex polymerase chain reaction in patients with COPD and healthy smoking control subjects.Data were analyzed with SPSS 10.0.Results The frequencies of the homozygous GST M1 null genotype in the cases and the controls were 58.9% and 52.3%,respectively.The frequencies of homozygous GST T1 null genotype in the two groups were 45.2% and 45.0%,respectively. The frequencies of combined GST M1 and GST T1 homozygous null genotype in the two groups were 27.4% and 19.3%,respectively.There are no significant differences in genetic polymorphism of GST M1 and GST T1 between the two groups.Conclusions Our results suggest that there are no associations of genetic polymorphism of either GST M1 or GST T1 with susceptibility to COPD.There is no marked synergism between the genotypes of GST M1 and GST T1.