| 介绍一种改良的麦康凯培养基 |
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| 引用本文： | 王继贵,童明华. 介绍一种改良的麦康凯培养基[J]. 中南大学学报(医学版), 1999, 24(3): 299-300 |
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| 作者姓名： | 王继贵 童明华 |
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| 作者单位： | 湖南医科大学,第二附属医院检验科,长沙,410011 |
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| 摘 要： | 用麦康凯（ＭＣＡ）培基粉作基础，加入６苯甲酰萘基磷酸二钠盐改良ＭＣＡ培养基。经多种肠道菌检测证实本法可同时观察乳糖和磷酸酶反应，在原始平板上根据菌落的颜色，直接区分肠杆菌科和非发酵菌之菌落，简化了鉴定手续，节约了鉴定时间，对进一步选择鉴定板有很大帮助。
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| 关 键 词： | 培养基 磷酸酶 非发酵菌 肠杆菌科 |
A modified MacConkey medium |
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| Wang Jigui,Tong Minghua. A modified MacConkey medium[J]. Journal of Central South University. Medical sciences, 1999, 24(3): 299-300 |
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| Authors: | Wang Jigui Tong Minghua |
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| Abstract: | We used MacConkey Agar(MCA) medium powder as a basis and added 6 benzoy 2 naphthyl phosphate disodium salt to modify MCA medium. This method was identified by examing several kinds of enteric pathogenic bacteria. Thus it allows us to observe lactose fermentative and phosphatase reaction simultaneously and to recognize Enterobacteriaceae from non fermentable bacteria on primary culture plates according to the color of colony. This improved method simplifies the recognition procedure and shortens the time so that it is helpful to correctly choosing identification kit. |
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| Keywords: | medium phosphatase non fermentable bacteria Enterobacteriaceae |
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