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| 快速灵敏的LC-MS/MS法测定人血浆中缬沙坦浓度 | |
| 引用本文： | 李玲,谭志荣,陈尧,周淦,郭栋,严谨,阳国平,欧阳冬生. 快速灵敏的LC-MS/MS法测定人血浆中缬沙坦浓度[J]. 中国临床药理学与治疗学, 2010, 15(7): 809-813 |
| 作者姓名： | 李玲 谭志荣 陈尧 周淦 郭栋 严谨 阳国平 欧阳冬生 |
| 作者单位： | 1. 中南大学临床药理研究所,长沙,410078,湖南;中南大学药学院,长沙,410078,湖南 2. 中南大学临床药理研究所,长沙,410078,湖南 3. 中南大学湘雅三医院,长沙,410013,湖南 |
| 摘 要： | 目的：建立一种快速、灵敏的高效液相色谱-串联质谱法（LC-MS/MS）测定人血浆缬沙坦浓度。方法：200μL血浆样品经乙腈一步沉淀蛋白后,在Inertsil ODS-色谱柱（2.1mm×150mm,5μm）上分离,流动相由乙腈和1‰甲酸水溶液（70∶30）组成。采用电喷雾离子源（ESI源）正离子多反应监测（MRM）扫描分析,缬沙坦和厄贝沙坦的离子选择通道分别为：m/z436.3→235.2和429.4→207.2。结果：缬沙坦的线性范围为24.2～3100.0μg/L,日内和日间相对标准差均小于15%。结论：本法操作快速、灵敏,适用于缬沙坦的临床药动学研究。 |
| 关 键 词： | 缬沙坦 液质联用法 生物等效性 |
A rapid and sensitive LC-MS/MS method for determination of valsartan in human plasma |
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| LI Ling,TAN Zhi-rong,CHEN Yao,ZHOU Gan,GUO Dong,YAN Jin,YANG Guo-ping,OUYANG Dong-sheng. A rapid and sensitive LC-MS/MS method for determination of valsartan in human plasma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 809-813 | |
| Authors: | LI Ling TAN Zhi-rong CHEN Yao ZHOU Gan GUO Dong YAN Jin YANG Guo-ping OUYANG Dong-sheng |
| Affiliation: | LI Ling1,2, TAN Zhi-rong1, CHEN Yao1, ZHOU Gan1, GUO Dong1, YAN Jin1, YANG Guo-ping3, OUYANG Dong-sheng11Institute of Clinic Pharmacology, Central South University, Changsha 410078,Hunan, China;2School of Pharmaceutics, Central South University, Changsha 410078, Hunan,China; 3The Third Xiangya Hospital, Central South University, Changsha 410013, Hunan,China |
| Abstract: | AIM：To establish a rapid and sensitive LC-MS/MS method for determination of valsartan in human plasma. METHODS： The prepared analytes were separated on an ODS column using mobile phase of acetonitrile and 1‰ methanoic acid （70∶30）. Valsartan and irbesartan were detected by multiple reaction monitoring （MRM） mode after ionized by ESI source. The transitions of m/z436.3→235.2 and m/z 429.4→207.2 were used to quantify valsartan and irbesartan, respectively. RESULTS：The method was linear in the range of 24.2-3100.0 μg/L for valsartan. The intra-day mean precision and inter-day mean accuracy precision was not more than 15%. CONCLUSION： The method is rapid and sensitive, and it＇s adaptive for clinic pharmacokinetic evaluation of valsartan. |
| Keywords: | Valsartan LC-MS/MS Bioequivalence |
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