A microassay for studies of the interaction between Fc receptors and low affinity ligands |
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| Authors: | M R Kepron A Froese |
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| Affiliation: | MRC Group in Allergy Research, Department of Immunology, University of Manitoba, Winnipeg, Manitoba R3E 0W3, Canada |
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| Abstract: | A microassay using 10 microliter of a conjugate of rat IgG and Sepharose was developed for the study of the interaction between the solubilized receptors for IgE of rat basophilic leukemia (RBL) cells, designated H and R, and a variety of low affinity immunoglobulins. Rat IgG2a was used as the model of a low affinity ligand by serving as an inhibitor of the interaction between IgG-Sepharose and the receptors for IgE. Ligand concentrations 2.24 X 10(-7) to 5.37 X 10(-5) M were used along with detergent extracts of 2 X 10(5) RBL cells. |
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