NSE和S-100蛋自在巨结肠同源病的表达

目的 探讨巨结肠同源病的病理形态学特征,提高其诊断符合率.方法 对131例行巨结肠根治术患儿的病变肠段进行常规HE染色,观察肠神经元及神经节细胞形态和数量的变化,与正常组进行比较,并对21例标本采用NSE和S-100蛋白进行免疫组织化学染色.结果 巨结肠同源病(HAD)标本的神经元和神经节细胞数与正常组及巨结肠症(HD)比较,差异有显著统计学意义(P＜0.01).结论 巨结肠同源病的诊断主要根据病理特征而定,在HE染色基础上结合NSE及S-100蛋白免疫组织化学染色能提高确诊率.

Expression of NSE and S-100 Protein Immunohistochemical Markers in Hirschsprung's Allied Disorder

Objective To study the pathological characteristics of Hirschsprung's disease allied disorder (HAD) by using NSE and S-100 protein immunohistochemical markers techniques, to improve the early diagnosis rate. Methods Intestinal segments from 131 children with HAD,who have undergoing radical operation were stained by HE convetional method. The morphological and quantity change of the neurone and ganglion cell of the lesion were observed and compared with the normal controls. NSE and S-100 protein immunohistochemical markers were stained in 21 samples. Results The number of the neurone and ganglion cell of HAD samples. was significantly higher than those in the normal control or HD group (P<0.01) . Conclusion The diagnosis of HAD is depended mainly on its pathologic characteristics. Introducing of NSE and S-100 protein immunohistochemical markers staining combined with traditional HE stain would be greatly helpful in making a definite diagnosis.