不同产地莲子心脂溶性成分的GC—MS分析

目的对7种不同产地莲子心中脂溶性成分进行GC-MS定性鉴别和指纹图谱分析。方法用氯仿提取莲子心的脂溶性成分，经甲酯化后，以气相-质谱联用技术（GC．MS）结合相对保留时间鉴定脂溶性的物质组成，采用面积归一化法测定它们在样品中的相对含量，并通过Excel相似度评价和SPSS聚类分析等统计手段，比较这7种莲子心脂溶性成分存在的差异。结果莲子心的40多种脂溶性成分被GC分离；16种被MS鉴定，其中有14种为脂肪酸类化合物，24种为酮类物质。采用2种色谱指纹图谱相似度计算法所得结果表明，不同产地的莲子心成分的相似度均在0.97以上；经对其脂溶性成分的逐步聚类分析，人为将这7种莲子心分为3类，但并未发现它们之间具有显著的因产地不同造成的区域性差异。结论初步认定7种不同产地莲子心的脂溶性成分具有高度一致性，在基本物质组成上并不存在地域的区别。 

GC-MS Analysis on Lipid Soluble Components in Plumula Nelumbinis Nuciferae Grown in Different Locations

OBJECTIVE To carry out GC-MS qualitative identification and fingerprint chromatography analysis on the lipid soluble components in Plumula Nelumbinis Nuciferae grown in different loeatious.METHOD Chloroform was used to extract the lipid soluble components of Plumula Nelumbinis Nuciferae, GC-MS technique was used to identify the composition of the lipid soluble substances, area nomalizatin method was used to determine the relative content of the substances in the sample, and Excel similarity evaluation and SPSS cluster analysis were used to compare the seven kinds of Phunula Nelumbinis Nuciferae. RESULT More than 40 kinds of lipid soluble components were isolated by GC; 16 kinds were identified by MS, in which 14 were fatty acids, 24 were ketone. The fingerprint chromatography analysis showed that the similarity rate of Plamula Nelumbinis Nueffeme grown in different locations was over 0.97. Seven kinds of Plumula Nelumbinis Nuciferae were divided into three classes according to cluster analysis, but no marked differences were detected among them. CONCLUSION The lipid soluble components of 7 kinds of Phumula Nelumbinis Nuciferae are of high consistency, with no area differences in basic composition.