| BA－ELISA快速检测鼻咽分泌物中合胞病毒抗原的研究 |
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| 引用本文： | 岳玉娟,孟红,秦立增,吴惠联,郭进林,李焱,王纪文,王兴步. BA－ELISA快速检测鼻咽分泌物中合胞病毒抗原的研究[J]. 现代免疫学, 1994, 0(2) |
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| 作者姓名： | 岳玉娟 孟红 秦立增 吴惠联 郭进林 李焱 王纪文 王兴步 |
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| 作者单位： | 山东省医学科学院基础所微生物室，山东医科大学附属医院，济南市第四人民医院 |
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| 摘 要： | 本文采用自制的ＲＳＶ免疫血清以及国产试剂，通过特异性、敏感度和重复性试验，建立了检测ＲＳＶ抗原的生物素－亲和素－酶联兔疫吸附试验（ＢＡ－ＥＬＩＳＡ）。该法比常规的ＥＬＩＳＡ法敏感４倍。用此法检测５８份肺炎和毛细支气管炎患儿鼻咽分泌物（ＮＰＳ）中的ＲＳＶ抗原，结果与组织培养（ＴＣ）分离ＲＳＶ结果比较：其特异性和敏感性分别为９１．２％和９５．８％，两法诊断符合率为９３．１％。结果表明本法不但敏感、特异，而且简便、快速、经济，在收到标本２４小时即可得到结果，是一种适应于基层推广的ＲＳＶ感染诊断方法。
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| 关 键 词： | RSV，BA－ELISA |
DETECTION OF RESPIRATORY SYNCYTIAL VIRUS ANTIGEN IN NASOPHARYNGEAL SECRETIONS BY BIOTIN-AVIDIN-ENZYME-LINKED-IMMUNOSORBENT ASSAY |
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| Yue Yujuan,Meng Hong,Qin Lizeng,Wu Rllillan et al. DETECTION OF RESPIRATORY SYNCYTIAL VIRUS ANTIGEN IN NASOPHARYNGEAL SECRETIONS BY BIOTIN-AVIDIN-ENZYME-LINKED-IMMUNOSORBENT ASSAY[J]. Current Immunology, 1994, 0(2) |
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| Authors: | Yue Yujuan Meng Hong Qin Lizeng Wu Rllillan et al |
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| Abstract: | A biotin-avidin-enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (RSV) antigens was developed, using antisera made in our institute. Guinea pig and rabbit anti--RSV immunoglobulins were uSed as capture and detector antibodies respectively.The specificity of this method was determined by crossing and blocking tests, revealing the sensitivity being 4 times higher than that ofconventional ELISA.Applying the assay to decteet RSV in 58 NPS specipsens, thespecficity and sensitivity were 91. ZN and 95.8% respectively.The result showed thatthe assay could be easily performed in 24h and was highly sensitiye and specific. |
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| Keywords: | RSV BA-ELISA |
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