荧光显微成像对于肝脏微循环的观察及相应肝固有动脉超选灌注动物模型的制作

目的探讨荧光显微成像对于研究肝内微循环的应用价值,总结适用于荧光显微技术的经肝固有动脉超选灌注动物模型的制作方法。方法应用10只Sprague-Dawley大鼠,开腹后,将微导管逆行置于胃十二指肠上动脉(GDA),导管开口朝向肝固有动脉的方向制成肝固有动脉超选灌注动物模型。经微导管分别注入0.02%、0.1%、0.5%、1%荧光素钠0.1ml后,应用荧光显微镜观察肝内微循环的显影情况,评价显影清晰度。经微导管分别注入栓塞剂后(碘油、直径40mm的可降解淀粉微球),观察其引发的微循环变化能否为荧光显微成像显示。结果10只Sprague-Dawley大鼠中,8只成功制成肝固有动脉超选灌注动物模型。荧光显微成像技术可以清晰的显示该动物模型肝内微循环的情况,0.1%的荧光素钠为最佳显影浓度。由栓塞剂引发的直接、间接现象均可为荧光显微成像技术所发现。结论荧光显微成像可以清晰显示肝内微循环结构,对于活体状态下肝脏微循环的形态学研究及栓塞剂的研发有高度的应用价值。适用于该技术的经肝固有动脉超选灌注动物模型的制作方法简单、成功率高,值得推广。

In vivo fluorescent microscopy of the liver microcirculation with a trans-proper hepatic arterial infusion animal model

Objective To evaluate the value of using in vivo fluorescent microscopy to study the liver microcirculation with a newly developed animal model for tans-proper hepatic arterial infusion. To summarize the method of making this animal model. Methods Ten Sprague-Dawley rats were used in this study. After a midline abdominal incision, microcatheter was placed into the gastroduodenal artery (GDA). The tip of the catheter was placed facing the orifice of proper hepatic artery. After infusion of 0.02%, 0.1%, 0.5%, 1% fluorescent sodium, fluorescent microscopy was used to evaluate the liver microcirculation. The image quality was accessed. Embolization was obtained by injection of lipiodol and degradable starch microspheres (DSM) from the microcatheter. Corresponding changes of the liver microcirculation was evaluated by fluorescent microscopy. Results Among the 10 rats, 8 animal models were successfully made. The microcirculation of the liver could be clearly visualized by the fluorescent microscopy. The optimal concentration of fluorescent sodium was 0.1%. The direct and indirect phenomena caused by embolic material could be evaluated by fluorescent microscopy. Conclusion Fluorescent microscopy with the corresponding trans-hepatic arterial infusion animal model is a valuable method to evaluate the microcirculation of the liver and helpful in the development of new type of embolic material.