| 幽门螺杆菌vacA及cagA基因型与胃疾病的关系 |
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| 引用本文： | 张凤娟,江月萍,赵清喜.幽门螺杆菌vacA及cagA基因型与胃疾病的关系[J].青岛大学医学院学报,2010,46(2):98-100. |
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| 作者姓名： | 张凤娟 江月萍 赵清喜 |
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| 作者单位： | 青岛大学医学院附属医院消化内科,山东,青岛,266003 |
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| 摘 要： | 目的探讨幽门螺杆菌(Hp)vacA、cagA基因型与胃疾病的关系。方法选取105例胃疾病病人,包括慢性浅表性胃炎(CSG)45例,慢性萎缩性胃炎(CAG)48例,胃癌(GC)12例。于胃窦处取3块胃黏膜,分别进行快速尿素酶反应、病理检查和聚合酶链反应(PCR)。提取胃黏膜基因组DNA,用6对引物检测Hp vacA、cagA基因的表达。结果快速尿素酶反应、病理检查均阳性的标本57例,Hp的阳性率为54.2%(57/105)。Hp vacA s1/m2、s1/m1、s2/m1、s2/m2的阳性率分别为53%(30/57)、18%(10/57)、8%(5/57)、21%(12/57);Hp cagA的阳性率为89%(51/57)。vacA、cagA基因型在CSG、CAG和GC之间差异无显著性(P>0.05)。结论Hp菌株的优势基因亚型为Hp cagA、vacA s1/m2;Hp vacA、cagA基因与特定胃疾病间无显著相关性,不能预示Hp感染的临床结果。
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| 关 键 词： | 胃疾病 螺杆菌 幽门 胃黏膜 vacA基因 cagA基因 聚合酶链反应 |
RELATIONSHIP BETWEEN GENOTYPES OF HELICOBACTER PYLORI vacA AND cagA AND GASTRIC DISEASES |
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| ZHANG FENG-JUAN,JIANG YUE-PING,ZHAO QING-XI.RELATIONSHIP BETWEEN GENOTYPES OF HELICOBACTER PYLORI vacA AND cagA AND GASTRIC DISEASES[J].Acta Academiae Medicinae Qingdao Universitatis,2010,46(2):98-100. |
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| Authors: | ZHANG FENG-JUAN JIANG YUE-PING ZHAO QING-XI |
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| Institution: | Department of Gastroenterology;The Affiliated Hospital of Qingdao University Medical College;Qingdao 266003;China |
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| Abstract: | Objective To investigate the relationship between vacA and cagA genotypes of Helicobacter pylori (Hp) and gastric diseases. MethodsThis study consisted of 105 patients with various gastric diseases including chronic superficial gastritis (CSG, 45 cases), chronic atrophic gastritis (CAG, 48), and gastric cancer (GC, 12). Three pieces of antrum mucosa were obtained from each patient. Rapid urease test, histological examination and polymerase chain reaction (PCR) were carried out in the specimen. DNA was extra... |
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| Keywords: | stomach diseases Helicobacter pylori gastric mucosa vacA gene cagA gene polymerase chain reaction |
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