CYP17A1和AR在脑胶质瘤中的表达及意义

目的探讨细胞色素P450c17a酶(CYP17A1)、雄激素受体(androgen receptor,AR)的表达与胶质瘤临床病理之间的相关性。方法应用免疫组化二步法检测55例胶质瘤组织和10例正常脑组织标本的CYP17A1蛋白和AR蛋白的表达情况。结果 1CYP17A1蛋白的表达在高级别胶质瘤和低级别胶质瘤之间具有差异性(χ~2=12.034,P0.05),而正常脑组织中未见表达。CYP17A1蛋白的表达与病理级别呈正相关(r=0.705,P0.05)。2AR蛋白的表达在高级别胶质瘤和低级别胶质瘤之间具有差异性(χ~2=19.509,P0.05),而正常脑组织中未见表达。AR蛋白的表达与病理级别呈正相关(r=0.73,P0.05)。结论CYP17A1蛋白及AR蛋白在脑胶质瘤组织中表达明显高于正常组织,且CYP17 A1蛋白及AR蛋白在胶质瘤组织中的表达情况与病理级别密切相关,提示雄激素轴在胶质瘤的发生发展中起到了重要的作用。

Expression of CYP17A1 and androgen receptor in glioma and its clinical significance

Objective To investigate the correlation between the expression of cytochrome P450c17α enzyme CYP17A1 and androgen receptor (AR) and the clinical pathology of glioma.Methods The two-step immunohistochemical method was used to measure the expression of CYP17A1 and AR proteins in 55 glioma tissue samples and 10 normal brain tissue samples.Results There was a significant difference in the expression of CYP17A1 protein between high-grade gliomas and low-grade ones (x2 =12.034,P ＜0.05),while there was no expression of CYP17A1 protein in normal brain tissue samples.The expression of CYP17A1 protein was positively correlated with pathological grade (r =0.705,P ＜ 0.05).There was a significant difference in the expression of AR protein between highgrade gliomas and low-grade ones (x2 =19.509,P ＜ 0.05),while there was no expression of AR protein in normal brain tissue samples.The expression of AR protein was positively correlated with pathological grade (r =0.73,P ＜ 0.05).Conclusions Glioma tissues have significantly higher expression of CYP17A1 and AR proteins than normal brain tissues,and the expression of CYP17A1 and AR proteins in glioma tissues are correlated with pathological grade,which suggests that the androgen axis plays an important role in the development and progression of glioma.