CD14+HLA-DRlow/-髓系来源抑制性细胞在Graves病中的检测及临床意义

目的:检测Graves病(Graves' disease,GD)患者外周血中髓系来源抑制性细胞(myeloid-derived suppressor cells,MDSCs)表达情况,探讨MDSCs在GD发生、发展中的作用.方法:收集52例初诊GD患者和30例健康志愿者外周血,以CD14+HLA-DR1ow/-作为MDSCs的免疫标记,分别应用流式细胞术及ELISA方法检测外周血中MDSCs的比例及血浆细胞因子Arg-1,IL-6,G-CSF浓度;并分析MDSCs与GD患者甲状腺功能相关性.结果:GD患者外周血MDSCs比例明显升高,相比对照组差异有统计学意义(P＜0.05),且GD患者病情平稳后,MDSCs水平较治疗前明显下降(P＜0.05);MDSCs水平与GD患者甲状腺功能未见明显相关性;GD患者外周血浆Arg-1水平未见明显升高,IL-6及G-CSF浓度显著升高(P＜0.05).结论:GD患者外周血MDSCs升高,可能是GD发生和发展的重要因素.

Detection and clinical significance of CD14+HLA-DRlow/-myeloid-derived suppressor cells in patients with Graves' disease

Objective:To investigate the expression and role of myeloid-derived suppressor cells (MDSCs) in the patients with Graves' disease (GD).Methods:A total of 52 newly diagnosed GD patients and 30 healthy controls were enrolled in the study.CD14+HLA-DR1ow/-was used as molecular markers for MDSCs in this study.The frequency of MDSCs in GD patients and healthy controls were determined by flow cytometry.ELISA was used for detecting the concentrations of plasma cytokines Arg-1,IL-6 and G-CSF.The correlation between the frequency of MDSCs and the function of thyroid was also analysed.Results:The frequency of MDSCs significantly increased in GD patients,compared with that in healthy controls.This frequency also obviously decreased in GD patients when treated with 131I (P＜0.05).There was no significant correlation between the frequency of MDSCs and the function of thyroid.The concentrations of plasma cytokines IL-6 and G-CSF significantly increased,in comparison with the healthy controls,while this was not existed in Arg-1.Conclusion:The increased frequency of MDSCs may play an important role in the occurrence and development of Graves' disease.