脓毒症小鼠死亡高发期腹腔脏器血流动力学观察

目的: 研究脓毒症小鼠死亡高发期腹腔脏器血流动力学的变化。方法: 应用高分辨小动物超声成像系统对正常雄性昆明小鼠先行心脏收缩功能和血流动力学指标的超声检测(0 h)后,行盲肠结扎穿孔术(CLP)。术后分5 个时点(12 h、24 h、36 h、48 h、60 h) 对小鼠进行相应超声指标检查。每只小鼠均进行编号,以便对小鼠进行连续性的观测,7 d仍存活的小鼠视为存活动物。结果: CLP小鼠在脓毒症死亡高发期心输出量(CO)大致保持在正常水平或高动力学状态。左心收缩功能出现明显的代偿性改变。腹主动脉血流量的变化表现为先升高后降低,其阻力指数(RI)和搏动指数(PI)从24 h 开始出现明显升高。右侧肾动脉的血流量从12 h开始便出现明显的下降,直到60 h仍明显低于正常,其RI和PI变化不明显。门静脉血流量12 h明显升高,24 h明显下降,其后血流量与0 h相比未见显著差异。充血指数从12 h即明显升高,其后维持在高于正常的水平。结论: 脓毒症小鼠腹腔各脏器在死亡高发期发生血流动力学的特征性变化。研究这些变化有助于阐明脓毒症的发病机制。

Hemodynamic changes of abdominal organs in the early stage of septic mice induced by cecal ligation and puncture

AIM:To characterize the hemoperfusion of abdominal organs in the early stage of sepsis in mice. METHODS:Health male Kunming mice were used in the study(n=100).The techniques of 2D,M-mode and pulse-wave Doppler were applied to evaluate the systolic functions of the heart and the blood flow of abdominal aorta,right renal artery and portal vein before cecal ligation and puncture(CLP)as the baseline and at the time points of 12 h,24 h,36 h, 48 h and 60 h after CLP.The mice survived for 7 d were considered as su...