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| 促红细胞生成素上调海马pCREB表达和改善脑缺血小鼠认知功能 | |
| 引用本文： | 陈远寿,潘贵书,秦伟,罗孝美,禹静,张弛. 促红细胞生成素上调海马pCREB表达和改善脑缺血小鼠认知功能[J]. 中国病理生理杂志, 2011, 27(4): 722-726. DOI: 10.3969/j.issn.1000-4718.2011.04.019 |
| 作者姓名： | 陈远寿 潘贵书 秦伟 罗孝美 禹静 张弛 |
| 作者单位： | 1. 遵义医学院生理教研室,贵州 遵义 563003; 2. 中国科学院神经科学研究所神经科学国家重点实验室,上海 200031 |
| 基金项目： | 中国科学院神经科学国家重点实验室开放课题资助项目 |
| 摘 要： | 目的: 探讨促红细胞生成素(EPO)对小鼠脑缺血所致的认知功能障碍和海马神经元损伤的保护作用及机制。方法: C57BL/6绿色荧光蛋白转基因小鼠随机分为假手术(sham)组、脑缺血/再灌注(I/R)组和EPO治疗组;采用双侧颈总动脉阻断(2-VO)方法复制小鼠全脑缺血模型,跳台实验测试小鼠学习记忆能力,Nissl染色检测海马神经元存活情况,Western blotting检测磷酸化cAMP反应元件结合蛋白(pCREB)表达水平,激光共聚焦显微镜和Neurolucida软件分析检测海马CA1区神经元形态及树突棘的变化。结果: 脑缺血导致小鼠学习记忆能力下降,海马CA1区神经元迟发性死亡和树突棘丢失;EPO治疗能显著提高脑缺血小鼠的学习记忆能力,减少脑缺血所致的海马CA1区神经元死亡和树突棘的丢失,显著上调海马CA1区神经元pCREB蛋白的表达。结论: EPO可能通过上调pCREB的表达来保护神经元损伤、防止神经元树突棘的丢失,进而改善脑缺血小鼠的认知功能。 |
| 关 键 词： | 脑缺血 红细胞生成素 cAMP反应元件结合蛋白质 树突棘 学习 记忆 |
| 收稿时间： | 2010-10-10 |
Erythropoietin attenuates cerebral ischemia-induced cognitive dysfunction through stimulation of hippocampal CREB phosphorylation in C57BL/6 mice |
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| CHEN Yuan-shou,PAN Gui-shu,QIN Wei,LUO Xiao-mei,YU Jing,ZHANG Chi. Erythropoietin attenuates cerebral ischemia-induced cognitive dysfunction through stimulation of hippocampal CREB phosphorylation in C57BL/6 mice[J]. Chinese Journal of Pathophysiology, 2011, 27(4): 722-726. DOI: 10.3969/j.issn.1000-4718.2011.04.019 | |
| Authors: | CHEN Yuan-shou PAN Gui-shu QIN Wei LUO Xiao-mei YU Jing ZHANG Chi |
| Affiliation: | 1. Department of Physiology, Zunyi Medical College, Zunyi 563003, China; 2. Institute of Neuroscience, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China |
| Abstract: | AIM:To investigate the neuroprotective effect of erythropoietin(EPO)on cognitive dysfunction and neuronal injury in hippocampal CA1 region induced by cerebral ischemia in mice.METHODS:Male C57BL/6 green fluorescent protein-transgenic mice were randomly divided into 3 groups;sham operation group(sham),ischemia/reperfusion group(I/R)and EPO-treated group.Transient cerebral global ischemia was induced by bilateral common carotid artery occlusion(2-VO).The step-down test was used to measure the capacity of lear... |
| Keywords: | Brain isehemia Erythropoietin cAMP response element -binding protein Dendritic spine Learning Memory |
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