siRNA下调astrocyteelevatedgene-1对神经母细胞瘤细胞增殖和凋亡影响的体外研究

目的: 探讨siRNA下调astrocyte elevated gene-1( AEG-1 )表达对神经母细胞瘤细胞增殖、凋亡和细胞周期的影响。方法: 用设计合成的AEG-1 siRNA转染神经母细胞瘤细胞株M17和SK-N-SH,采用荧光定量RT-PCR技术观察AEG-1 siRNA对 AEG-1 基因表达的影响;用MTT法和克隆形成实验观察AEG-1 siRNA对神经母细胞瘤细胞的增殖抑制作用;用流式细胞术检测下调 AEG-1 表达对神经母细胞瘤细胞凋亡及细胞周期的影响。结果: 用AEG-1 siRNA转染人神经母细胞瘤细胞,RT-PCR结果证实AEG-1 siRNA能显著基因下调 AEG-1 表达,与对照组相比有显著差异(P<0.01);MTT法和克隆形成实验结果证明下调 AEG-1 表达可显著抑制神经母细胞瘤细胞的增殖;流式细胞术检测结果表明下调 AEG-1 表达可促进神经母细胞瘤细胞凋亡,并将细胞周期阻滞在G₀/G₁期。结论: AEG-1 siRNA可下调基因在神经母细胞瘤细胞中表达,并抑制神经母细胞瘤细胞增殖,促进细胞凋亡。

Knockdown of astrocyte elevated gene-1 by siRNA inhibits cell proliferation and induces apoptosis in human neuroblastoma cell line

AIM:To investigate the effect of siRNA-induced astrocyte elevated gene-1(AEG-1)down-regulation on the proliferation,apoptosis and cell cycle of neuroblastoma cells.METHODS:An siRNA targeting to AEG -1 mRNA(AEG-1 siRNA)was constructed and transfected into neuroblastoma cells with Lipofectamine 2000.A non-specific siRNA(control siRNA)and non-treatment were used as negative control and blank control,respectively.The cell proliferation was detected by MTT method and colony formation assay.The apoptosis and cell...