|
|||||
|
|
| 营养性肥胖大鼠的脂代谢紊乱与下丘脑中增食欲素A的表达分析 | |
| 引用本文： | 赵玉红,吴丽霞,关心,王伟,赵玉岩. 营养性肥胖大鼠的脂代谢紊乱与下丘脑中增食欲素A的表达分析[J]. 中国病理生理杂志, 2011, 27(5): 1000-1002. DOI: 10.3969/j.issn.1000-4718.2011.05.032 |
| 作者姓名： | 赵玉红 吴丽霞 关心 王伟 赵玉岩 |
| 作者单位： | 1. 沈阳医学院沈洲医院检验科,辽宁 沈阳 110002; 2. 中国医科大学附属第一医院内分泌科,辽宁 沈阳 110001 |
| 基金项目： | 国家自然科学基金资助项目，辽宁省自然科学基金资助项目，辽宁省科技攻关层次计划资助项目 |
| 摘 要： | 目的: 研究营养性肥胖大鼠下丘脑中增食欲素(orexin A)表达与脂代谢紊乱的规律。 方法: 高脂饮食诱导并评估营养性肥胖大鼠动物模型;采用化学发光免疫分析法和生化酶法测定大鼠血清胰岛素(Ins)、甘油三酯(TG)和总胆固醇(TC);应用实时定量PCR检测下丘脑组织中orexin A mRNA的表达规律。 结果: 大鼠高脂膳食饲养8周后,营养性肥胖大鼠的体重、体脂含量和Lee's指数均高于对照组,血清Ins增加约50％,TG和TC分别增加约94％和43％(P<0.05);营养性肥胖大鼠下丘脑中orexin A的mRNA表达减少约57 % (P<0.05), 且orexin A的表达量与Lee's指数、Ins、TG、TC呈显著负相关,相关系数分别为r=-0.798 (P<0.05)、r=-0.868(P<0.05)、r=-0.981(P<0.05)和r=-0.815(P<0.05)。结论: 在大鼠高脂膳食诱导的营养性肥胖过程中,下丘脑orexin A的低表达和脂代谢紊乱均与营养性肥胖的发生密切相关。 |
| 关 键 词： | 增食欲素A 肥胖症 脂代谢 基因表达 |
| 收稿时间： | 2010-05-31 |
Hypothalamic expression of orexin A and lipid metabolism disorder in rats with alimentary obesity |
|
| ZHAO Yu-hong,WU Li-xia,GUAN Xin,WANG Wei,ZHAO Yu-yan. Hypothalamic expression of orexin A and lipid metabolism disorder in rats with alimentary obesity[J]. Chinese Journal of Pathophysiology, 2011, 27(5): 1000-1002. DOI: 10.3969/j.issn.1000-4718.2011.05.032 | |
| Authors: | ZHAO Yu-hong WU Li-xia GUAN Xin WANG Wei ZHAO Yu-yan |
| Affiliation: | 1. Department of Laboratory Medicine, The Second Affiliated Hospital of Shenyang Medical College, Shenyang 110002, China; 2. Department of Endocrinology, First Clinical Hospital, China Medical University, Shenyang 110001, China |
| Abstract: | AIM: To evaluate the effect of orexin A in rat hypothalamus on lipid metabolism disorder in rats with alimentary obesity induced by high-fat diet.METHODS: The rat model of alimentary obesity was induced by high-fat diet.The levels of insulin,triglyceride(TG) and total cholesterol(TC) in the serum were detected by luminescent immunoassay and enzymic method.The mRNA expression of orexin A in rat hypothalamus was determined by real-time PCR.RESULTS: There were statistically significant differences of weight,bo... |
| Keywords: | Orexin A Obesity Lipid metabolism Gene expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国病理生理杂志》浏览原始摘要信息 | |
| 点击此处可从《中国病理生理杂志》下载免费的PDF全文 | |