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| 人脐血MSCs向神经元样细胞分化过程中neurogenin1的表达变化 | |
| 引用本文： | 朱登纳,贾延劼,王军,张博爱,范亚珍. 人脐血MSCs向神经元样细胞分化过程中neurogenin1的表达变化[J]. 中国病理生理杂志, 2011, 27(5): 996. DOI: 10.3969/j.issn.1000-4718.2011.05.031 |
| 作者姓名： | 朱登纳 贾延劼 王军 张博爱 范亚珍 |
| 作者单位： | 1. 郑州大学 第三附属医院河南省小儿脑瘫康复治疗中心,河南 郑州 450052; 2. 郑州大学 第一附属医院神经内科,河南 郑州 450052 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的: 探讨人脐血间质干细胞(MSCs)体外向神经元样细胞分化过程中neurogenin 1的表达变化。方法: 常规从人脐血中分离MSCs,采用生长因子EGF和bFGF联合诱导人脐血MSCs向神经元样细胞分化,免疫细胞化学法检测诱导前、后神经元表面标志蛋白NF-M、NSE、胶质细胞标志蛋白GFAP和neurogenin 1的表达情况;Western blotting和RT-PCR法检测诱导前、后人脐血MSCs中neurogenin 1蛋白和mRNA的表达变化。结果: 诱导前,人脐血MSCs不表达NF-M、NSE和GFAP;诱导7 d,人脐血MSCs可以分化为神经元样细胞, 同时表达NF-M、NSE和GFAP。诱导前,人脐血MSCs不表达neurogenin 1,诱导后neurogenin 1蛋白和mRNA表达显著增加。结论: Neurogenin 1可能参与了体外诱导人脐血MSCs向神经细胞分化的过程。 |
| 关 键 词： | 神经元素1 脐带血 间质干细胞 神经元 |
| 收稿时间： | 2010-10-21 |
Expression of neurogenin 1 in MSCs isolated from human umbilical cord blood during differentiation into neuron-like cells |
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| ZHU Deng-na,JIA Yan-jie,WANG Jun,ZHANG Bo-ai,FAN Ya-zhen. Expression of neurogenin 1 in MSCs isolated from human umbilical cord blood during differentiation into neuron-like cells[J]. Chinese Journal of Pathophysiology, 2011, 27(5): 996. DOI: 10.3969/j.issn.1000-4718.2011.05.031 | |
| Authors: | ZHU Deng-na JIA Yan-jie WANG Jun ZHANG Bo-ai FAN Ya-zhen |
| Affiliation: | 1. Rehabilitation and Treatment Center for Child Cerebral Palsy, The Third Affiliated Hospital,Zhengzhou University, Zhengzhou 450052, China; 2. Department of Neurology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China |
| Abstract: | AIM: To observe the expression of neurogenin 1 in mesenchymal stem cells(MSCs) during the process of differentiation into neuron-like cells.METHODS: The MSCs were isolated from human umbilical cord blood,then induced and differentiated into neuron-like cells in the medium containing EGF and bFGF in vitro.NF-M,NSE,GFAP and neurogenin 1,the specific neuron antigen markers,were detected by immunohistochemistry method in cultured cells before and after induction.The expression of neurogenin 1 at mRNA and protei... |
| Keywords: | neurogenin 1 Umbilical cord blood Mesenchymal stem cells Neurons |
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