复方丹参注射液抗脂多糖诱导的兔弥漫性血管内凝血

目的: 研究复方丹参注射液对脂多糖(LPS)诱导的兔弥漫性血管内凝血(DIC)的作用。方法: 用LPS诱导兔DIC模型。凝固法测定纤维蛋白原含量,全自动凝血分析仪检测活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)和纤维蛋白原含量;全自动血细胞分析仪进行血小板计数;全自动血浆分析仪测定谷丙转氨酶(ALT)以及血尿素氮(BUN);发色底物法测定蛋白C及抗凝血酶Ⅲ的活性。观察复方丹参注射液对LPS诱导的兔DIC的拮抗作用。结果: 兔耳缘静脉持续滴注LPS,观察到:APTT和PT显著延长;血小板计数和纤维蛋白原含量明显减少;ALT和BUN显著升高;蛋白C和抗凝血酶Ⅲ的活性明显降低。给予复方丹参注射液后,APTT和PT的延长明显缩短;血小板计数和纤维蛋白原的含量均明显恢复;ALT和BUN显著下降;蛋白C及抗凝血酶Ⅲ的活性显著改善。结论: 复方丹参注射液对LPS诱导的兔DIC有良好的拮抗作用。

Effect of compound Salvia militorrhiza injection on LPS-induced disseminated intravascular coagulation in rabbits

AIM: To evaluate the effect of compound Salvia militorrhiza(SM) injection on lipopolysaccharide (LPS)-induced experimental disseminated intravascular coagulation (DIC) in rabbits. METHODS: The LPS-induced DIC model in rabbits was performed by continuous infusion of LPS at 100 μg·kg^-1·h^-1 for 6 h. Treatment with compound SM injection or heparin was begun simultaneously with LPS infusion through the contralateral marginal ear vein.Activated partial thromboplastin time (APTT), prothrombin time (PT), platelet count and fibrinogen concentration were determined. The plasma levels of alanine aminotransferase (ALT) and blood urine nitrogen (BUN) were detected. The activity of protein C and antithrombi III (ATIII) was also measured.RESULTS: Continuous infusion of LPS induced gradual impairment of hemostatic parameters and damages in renal and liver functions. The intravenous administration of compound SM injection attenuated the increase in plasma levels of APTT and PT, and the decrease in plasma levels of fibrinogen and platelet induced by LPS infusion. Compound SM injection decreased the levels of ALT and BUN, reduced the damages in the liver and kidney, and also significantly inhibited the decreased plasma levels of protein C and ATIII in LPS- treated animals.CONCLUSION: Compound SM injection has a protective effect against DIC in rabbits.