激光多普勒血流仪评价活体大鼠大脑中动脉栓塞模型成功的可行性分析

目的：探讨激光多普勒血流仪（LDF）评价活体大鼠脑缺血/再灌注损伤模型制作成功的可行性和可靠性分析。方法：10只体重280-310 g的雄性SD大鼠用传统线栓法制作单侧大脑中动脉阻塞（MCAO）模型，神经功能评分，LDF监测插栓前后大鼠脑血流动态变化。于缺血2 h和再灌注24 h断头取脑，TTC染色检测脑片梗死灶体积。结果：基线血流均值为 (224.99±75.00) PU，局灶神经功能障碍评分0分。插栓成功MCAO模型，缺血2 h血流均值为 (67.23±6.90) PU，与基线血流差值为157.76PU，降幅约为70%（P<0.01），差异显著。拔拴后血流均值为 (216.01±7.30) PU，与缺血2 h血流差值为148.78PU（P＜0.01），差异显著；与基线血流差值为-8.98 PU（P>0.05），无显著差异。再灌注24 h后，局灶神经功能障碍评分10.35分，脑片TTC染色显示梗死及水肿灶。插栓不成功模型LDF监测显示仍有血流通过，降幅少于基线的50%，再灌注24 h后，局灶神经功能障碍评分0分，TTC染色未见梗死灶。结论：LDF对大鼠脑血流的监测是除神经功能评分以外另一种判断活体大鼠MCAO模型制作成功的可靠而实用的方法。

Evaluation of rat MCAO model by laser Doppler flowmetry

AIM: To investigate the feasibility and reliability of using laser Doppler flowmetry(LDF) to evaluate the animal model of cerebral ischemia and reperfusion injury in vivo.METHODS: Ten Sprague-Dawley(SD) male rats,weighing 280-310 g,were subject to unilateral middle cerebral artery occlusion(MCAO) with the routine method of line-embolism,and the cortical blood flow was continuously monitored by LDF during ischemia and reperfusion in the rats.Meanwhile,the degree of injury induced by cerebral ischemia and rep...