氢气对慢性低氧高二氧化碳大鼠学习记忆障碍的干预作用

目的: 观察氢气(H₂)对慢性低氧(O₂)高二氧化碳(CO₂)模型大鼠学习记忆的干预作用。方法: 将通过八臂迷宫训练的24只清洁级雄性SD大鼠随机分为常氧对照组(NC)、低O₂高CO₂+生理盐水组(MS)、低O₂高CO₂+H₂(MH),每组8只。NC组于常温常压条件下饲养,MS组和MH组置于O₂浓度9％-11％ 和CO₂ 浓度5％-6％的舱内饲养,每天8 h,每周6 d,持续4周,其余时间饲养条件与NC组相同。每天出舱后,MS组腹腔注射生理盐水,MH组腹腔注射饱和H₂水,剂量均为5 mL/kg。4周后,八臂迷宫测试3组大鼠的学习记忆能力;酶联免疫吸附法检测海马8-羟基脱氧鸟苷(8-OHdG)含量;分光光度计检测血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;显微镜下观察3组大鼠海马超微结构及神经元凋亡小体数量。结果: (1)与NC组比较,MS组和MH组工作记忆错误(WME)次数增加,海马8-OHdG和血清MDA含量增加,血清SOD活性降低,海马部位细胞结构排列紊乱,尼氏体形状和数量均有改变,凋亡细胞明显增多。(2)与MS组相比,MH组WME次数减少,海马8-OHdG和血清MDA含量降低,血清SOD活性增加,细胞结构较整齐,尼氏体丰富完整,凋亡神经元数量明显减少。结论: H₂改善低O₂高CO₂大鼠学习记忆障碍,可能与减少神经元凋亡和氧化应激损伤有关。

Intervention of hydrogen on memory damage induced by chronic hypoxia-hypercapnia in rats

AIM: To investigate the effects of hydrogen on the memory damage caused by chronic hypoxia-hypercapnia in rats.METHODS: Twenty-four SD rats trained by eight-arm radial maze test were randomly divided into 3 groups:normal control group(NC),hypoxia-hypercapnia+saline group(MS) and hypoxia-hypercapnia+ hydrogen group(MH).The rats in the latter 2 groups were placed in a closed cabin for 8 h/day,6 days/week and lasted for 4 weeks,in which O2 was 9%-11% and CO2 was 5%-6%.In every time after the animals were out o...