2型糖尿病肾损害大鼠模型的建立

目的 在原有高糖高脂饮食+链脲佐菌素(STZ)造模方法的基础上进一步缩短造模时间,优化模型,使模型更加接近于人类2型糖尿病肾脏病,为探索其机制及治疗方法提供一个更好的动物模型.方法 将4周的雄性SD大鼠,随机分为正常饲料组(A组)和高糖高脂饲料+STZ组(B组).B组采用高糖高脂饲料+5%葡萄糖饮水喂养+STZ,诱发胰...

Establishment of rat models of type 2 diabetic kidney injury

Objective To optimize a rodent model of type 2 diabetes kidney injury that would replicate the metabolic characteristics of the human syndrome. Methods Four-week age male SD rats were randomly divide into conventional diet group （n = 15）, high glucose group, and high fat diet with streptozotocin （STZ） group （n = 15）. Rats were fed two weeks on the diets enriched with sucrose （10% w/w）, lard （12% w/w） and egg （10% w/w） to induce FINS resistance. Hyperglycemia was developed by intraperitoneal injection of STZ （30 mg/kg） in these rats three times within consecutive three days. Rats were observed for 6 months after STZ injection. The levels of body weight, urine protein, urine albumin, blood glucose were determined. Blood samples were collected for serum crea- tine, BUN, serum FINS before and 6 weeks after injectiom Results There was no significant difference in body weight, blood glucose, serum FINS, serum creatine and BUN before interference among the groups （P〉0. 05）. Serum FINS in high fat and high glucose diet with STZ groups was significantly increased after 2 weeks of diet interference （P〈0. 01）. Blood glucose levels were significantly increased after 72 h of injection （P〈0. 01）. Clinically there was continuous proteinuia. Pathologically, there were proliferation of mesangial cells, increase of mesangial matrix and thickening of glomerular basement membrane. Conclusions Rat model of type 2 diabetic kidney injury can be successfully developed by high fat and high glucose diet with low dose of STZ.