三七总皂苷和淫羊藿苷组分配伍对高糖培养成骨细胞增殖功能的影响

目的:观察三七总皂苷和淫羊藿苷组分配伍对高糖条件下成骨细胞增殖功能的影响.方法:取3～5代成骨细胞进行试验,成骨细胞(OB)密度为1×105个/mL,观察葡萄糖(Glu)浓度分别为20,40,60 mmol·L-1时成骨细胞的生长情况.三七总皂苷和淫羊藿苷质量浓度为10,20,50,100 mg·L-1,采用MTT法观察单剂量药物及其配伍对高糖条件下成骨细胞的增殖抑制的改善情况.结果:与对照组相比,60 mmol· L-1 Glu的高糖环境对于OB的增殖有明显的抑制作用(P ＜0.05,P＜0.01);三七总皂苷和淫羊藿苷在质量浓度为10,20,50 mg·L-1时对高糖引起的OB增殖抑制具有显著改善作用(P ＜0.05,P＜0.01),组分配伍比例三七总皂苷:淫羊藿苷为50 mg·L-1∶20 mg·L-1时,对高糖条件下成骨细胞增殖抑制具有明显的改善作用(P＜0.01).结论:从对高糖条件下成骨细胞增殖抑制改善作用的角度证实了三七总皂苷和淫羊藿苷组分配伍的合理性.

Effect of the Compatibility of Panax notoginseng Saponinsn and Icariin on Proliferation of Osteoblasts Cultured by High Glucose

Objective: To explore the effects of the compatibility of Panax notoginseng saponinsn and icariin on high glucose-induced proliferation inhibition of osteoblasts. Method: The third-fifth generation cells were taken as experimental model, osteoblast density was 1×10⁵/mL,the growth of the osteoblasts was observed when glucose (Glu) concentrations were 20,40,60 mmol·L^-1.The concentration of P. notoginseng saponins and icariin were 10,20,50,100 mg·L^-1,MTT was applied to determine the protective effect of high glucose-induced proliferation inhibition of osteoblasts. Result: Compared with control group, the growth of the osteoblasts was significantly inhibited when Glu concentrations was 60 mmol·L^-1 (P<0.05,P<0.01).P. notoginseng saponins and icariin in a dose of 10,20,50 mg·L^-1 had significantly protective effect of high glucose-induced proliferation inhibition of osteoblasts(P<0.05,P<0.01).When the component formula of P. notoginseng saponinsn and icariin was 50 mg·L^-1: 20 mg·L^-1,compared with control group,the component group can promote proliferation of osteoblasts(P<0.01). Conclusion: The compatibility of P. notoginseng saponinsn and icariin was feasible in the protective effect of high glucose-induced proliferation inhibition of osteoclasts.