| 华蟾素注射液对人肝癌HepG-2细胞增殖及拓扑异构酶表达的影响 |
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| 引用本文： | 高山,田莉莉,陈华,崔晓楠. 华蟾素注射液对人肝癌HepG-2细胞增殖及拓扑异构酶表达的影响[J]. 中国实验方剂学杂志, 2013, 19(11): 250-255 |
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| 作者姓名： | 高山 田莉莉 陈华 崔晓楠 |
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| 作者单位： | 大连医科大学第一附属医院肿瘤科,辽宁大连,116011 |
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| 基金项目： | 国家自然科学基金项目(81173615) |
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| 摘 要： | 目的:探讨华蟾素注射液对人肝癌HepG-2细胞增殖及拓扑异构酶Ⅰ(Topo Ⅰ)和Ⅱ的影响.方法:实验分为对照组(不加药)与实验组(华蟾素注射液0.105,0.21,0.42 mg·L-1)；采用四甲基偶氮唑蓝还原法(MTT)观察对HepG-2细胞增殖的影响；采用流式细胞术(AnnexinV/PI)双染色法检测对HepG-2细胞凋亡的影响；采用流式细胞术法检测对HepG-2细胞周期的影响；采用逆转录-多聚酶联反应(RT-PCR)法检测对肝癌HepG-2细胞Topo Ⅰ,TopoⅡmRNA水平表达的影响；采用蛋白免疫印迹法(Western-blot法)观察对HepG-2细胞中Topo Ⅰ,TopoⅡ蛋白表达的影响.结果:华蟾素注射液抑制HepG-2细胞增殖(P＜0.05),诱导细胞凋亡(P＜0.05),呈时间和剂量依赖性；阻滞HepG-2细胞于S期(P＜0.05),呈剂量依赖性；下调Topo Ⅰ,TopoⅡmRNA(P ＜0.05)及蛋白的表达(P＜0.05),呈剂量依赖性.结论:华蟾素注射液能够抑制人肝癌HepG-2细胞增殖,诱导癌细胞凋亡,下调Topo Ⅰ,TopoⅡ表达可能是其机制之一.
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| 关 键 词： | 华蟾素注射液 HepG-2细胞 增殖 凋亡 拓扑异构酶Ⅰ 拓扑异构酶Ⅱ |
| 收稿时间： | 2012-09-07 |
Effects of Cinobufacini Injections on Proliferation and Expression of Topoisomerase of Hepatocarcinoma HepG-2 Cells |
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| GAO Shan,TIAN Li-li,CHEN Hua and CUI Xiao-nan. Effects of Cinobufacini Injections on Proliferation and Expression of Topoisomerase of Hepatocarcinoma HepG-2 Cells[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(11): 250-255 |
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| Authors: | GAO Shan TIAN Li-li CHEN Hua CUI Xiao-nan |
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| Affiliation: | Department of Oncology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;Department of Oncology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;Department of Oncology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;Department of Oncology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China |
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