| 在线紫外光谱对栀子及其炮制品HPLC指纹图谱的定性研究 |
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| 引用本文： | 侯志飞,于文国,王刚,陈晶,孙国祥.在线紫外光谱对栀子及其炮制品HPLC指纹图谱的定性研究[J].中国实验方剂学杂志,2013,19(11):98-101. |
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| 作者姓名： | 侯志飞 于文国 王刚 陈晶 孙国祥 |
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| 作者单位： | 1. 河北化工医药职业技术学院,石家庄,050026
2. 沈阳药科大学药学院,沈阳,110016 |
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| 基金项目： | 河北省中医药管理局科研项目(2011109) |
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| 摘 要： | 目的:对栀子及其炮制品HPLC指纹图谱指纹峰进行定性归属研究.方法:采用反相高效液相色谱法,使用CenturySIL C18 BDS柱(4.6 mm×200mm,5μm),以1％醋酸水-1％醋酸乙腈为流动相,低压线性梯度洗脱,检测波长265 nm,柱温(30.0±0.15)℃,进样量5μL,测定栀子及其炮制品的HPLC指纹图谱,并应用DAD检测器记录指纹峰在线紫外光谱.结果:以栀子苷峰为参照物峰,确定35个共有峰,根据各指纹峰紫外光谱特点进行定性归属,并计算栀子及其炮制品中各类指纹峰的面积归一化值.结论:环烯醚萜苷类、有机酸酯类和西红花苷类成分是栀子的主要成分,但在不同产地栀子中各类成分所占比例有一定差异,栀子经炮制后各类成分所占比例也发生了变化.
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| 关 键 词： | 在线紫外光谱 指纹峰定性归属 栀子及其炮制品 高效液相色谱指纹图谱 |
| 收稿时间： | 2012/7/23 0:00:00 |
Study on Qualitative Belonging of Fingerprint Peaks of HPLC Fingerprints for Gardeniae Fructus and the Prepared Medical Materials |
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| HOU Zhi-fei,YU Wen-guo,WANG Gang,CHEN Jin and SUN Guo-xiang.Study on Qualitative Belonging of Fingerprint Peaks of HPLC Fingerprints for Gardeniae Fructus and the Prepared Medical Materials[J].China Journal of Experimental Traditional Medical Formulae,2013,19(11):98-101. |
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| Authors: | HOU Zhi-fei YU Wen-guo WANG Gang CHEN Jin and SUN Guo-xiang |
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| Institution: | Pharmaceutical Engineering Department, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China;Pharmaceutical Engineering Department, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China;Pharmaceutical Engineering Department, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China;Pharmaceutical Engineering Department, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China;College of pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | |
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| Keywords: | UV-spectrum qualitative belonging Gardeniae Fructus and the prepared medical materials HPLC digital fingerprint |
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