| 高速逆流色谱法分离鉴定苗药三两银中的甾体生物碱 |
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| 引用本文： | 何康,王进喜,杜江.高速逆流色谱法分离鉴定苗药三两银中的甾体生物碱[J].中国实验方剂学杂志,2013,19(11):137-139. |
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| 作者姓名： | 何康 王进喜 杜江 |
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| 作者单位： | 贵阳中医学院,贵阳,550002 |
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| 基金项目： | 国家自然科学基金项目(30960529);贵阳中医学院博士启动基金项目(201208) |
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| 摘 要： | 目的:研究苗药三两银中的生物碱类成分.方法:应用高速逆流色谱法,以正己烷-乙酸乙酯-无水乙醇-水(6∶1.2∶7.1∶1)上相为固定相,下相为流动相,组成溶剂系统对苗药三两银中的总碱进行分离纯化及鉴定.结果:从其总碱部分分离得到3个甾体类生物碱,根据理化性质和光谱数据鉴定为:海南野扇花碱D (sarcovagine D,1)、富贵草碱G(physamine G,2)和富贵草碱H (physamine H,3).结论:首次从该种植物中分离得到化合物2,3.
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| 关 键 词： | 高速逆流 甾体生物碱 羽脉野扇花 |
| 收稿时间： | 2012/12/14 0:00:00 |
Steroidal Alkaloids from Sarcococca hookeriana by High-speed Countercurrent Chromatography |
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| HE Kang,WANG Jin-xi and DU Jiang.Steroidal Alkaloids from Sarcococca hookeriana by High-speed Countercurrent Chromatography[J].China Journal of Experimental Traditional Medical Formulae,2013,19(11):137-139. |
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| Authors: | HE Kang WANG Jin-xi and DU Jiang |
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| Institution: | Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China |
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| Abstract: | Objective: To study the steroidal alkaloids from Sarcococca hookeriana. Method: High-speed counter-current chromatography (HSCCC) was employed first to separate and purify compounds.A biphasic solvent system composed of n-hexane-EtAc-dehydrated alcohol-water (6: 1.2: 7.1: 1) was used. Result: Three steroidal alkaloids were isolated from the total alkaloids and their structure were identified as:sarcovagine D (1), physamine G (2) and physamine H (3). Conclusion: Compounds 2 and 3 were isolated from this plant for the first time. |
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| Keywords: | high-speed countercurrent chromatography steroidal alkaloids Sarcococca hookeriana |
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