S100A4蛋白和上皮钙黏蛋白-E在黑素瘤中的表达及意义

目的探讨黑素瘤的发生和侵袭转移机制,检测S100A4蛋白、上皮钙黏蛋白-E(E-cadherin)在黑素瘤的表达水平。方法应用免疫组织化学SP法检测30例黑素瘤、30例黑素细胞痣和20例正常皮肤组织中S100A4和E-cadherin蛋白的表达。结果 S100A4在黑素瘤组织中表达的阳性率为66.67%,明显高于其在黑素细胞痣中的表达(13.33%),在正常皮肤组织中基本不表达,两者比较差异有统计学意义(P0.05);E-cadherin在正常皮肤组织表达率为100%,与在黑素细胞痣中的表达(83.3%)差异无统计学意义,在黑素瘤组织中表达率为43.33%,与正常皮肤组织比较有统计学意义(P0.05)。在有淋巴转移和无淋巴转移的黑素瘤中S100A4和E-cadherin的表达差异均有统计学意义(P0.05)。结论 S100A4和E-cadherin与黑素瘤的转移可能有关,提示黑素瘤组织中S100A4和E-cadherin的检测可作为预测肿瘤转移的指标之一。

Expression and Significance of S100A4, E-cadherin in Malignant Melanoma

Objective To explore the molecular mechanism of human malignant melanoma,and expression of S100A4 and E-cadherin in malignant melanoma and provide theoretic basis for tumorigenesis mechanism and gene therapy. Methods The expression of S100A4 and E-cadherin in 30 malignant melanoma,30 melanocytic nevus and 20 normal skin were detected by SP immunohistochemistry. Results Positive rates of S100A4 in malignant melanoma(66.67%)were significantly higher than that in normal skin and melanocytic nevus(13.33%). Positive rates of E-cadherin in malignant melanoma(43.33%)were lower than that in normal skin(100%) and melanocytic nevus.Expression of S100A4 and E-cadherin in metastasize malignant melanoma were greatly different from the ones without metastasis. Conclusion S100A4 and E-cadherin are closely related to the metastasis of malignant melanoma,and may be the predictors of the metastasis.