独活寄生汤水提物对软骨细胞G1期调控因子mRNA表达的影响

目的: 探讨独活寄生汤水提物对软骨细胞G₁期的影响。 方法: 取4周龄雄性SD大鼠,建立软骨细胞体外培养体系,第3代软骨细胞进行干预,采用MTT法检测不同浓度的独活寄生汤水提物对软骨细胞活性的影响;RT-PCR检测空白组、独活寄生汤水提物低、中、高剂量组(100,200,400 mg·L^-1)软骨细胞Cyclin D1,CDK4,CDK6及P21 mRNA的表达。 结果: MTT法检测显示,在一定的药物质量浓度范围内(50~800 mg·L^-1),软骨细胞活性显著上升(P<0.01);干预24 h后,Cyclin D1,CDK4及CDK6 mRNA表达量各剂量组明显增加(P<0.05),200 mg·L^-1表达量最大(P<0.01);P21 mRNA表达量降低,且200 mg·L^-1抑制作用最明显(P<0.01)。 结论: 独活寄生汤水提物通过影响软骨细胞G₁期调控因子mRNA的表达,促进软骨细胞的增殖。

Effect of water extracts from Duhuo Jisheng decoction on expression of chondrocyte G1 phase regulator mRNA

Objective: To investigate the effect of water extracts from Duhuo Jisheng decoction on chondrocyte G₁ phase. Method: Chondrocytes were collected from four-week-old SD rats to establish the chondrocyte in vitro culture system. The third generation of chondrocytes was intervened. MTT method was used to measure the effect of water extracts from different concentrations of Duhuo Jisheng decoction on chondrocyte activity. The expressions of Chondrocyte Cyclin D1, CDK4, CDK6 and P21 mRNA in the blank group and low, middle and high-dose groups (100, 200, 400 mg·L^-1) were detected by RT-PCR method. Result: The MTT assay showed that the chondrocyte activity significantly increased within specific drug concentrations (50-800 mg·L^-1) (P<0.01);After the intervention for 24 h, the expressions of CyclinD1, CDK4 and CDK6 mRNA in all dose groups notably increased (P<0.05), with the maximum expressions at the concentration of 200 mg·L^-1;The expression of P21 mRNA decreased, particularly at the concentration of 200 mg·L^-1 (P<0.01). Conclusion: Water extracts from Duhuo Jisheng decoction can promote chondrocyte proliferation by effecting the expression of chondrocyte G₁ phase regulator mRNA.