| 试剂及血清中丙酮酸对ALT测定结果的影响及其消除方法 |
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| 引用本文： | 王春新 杨荣伟. 试剂及血清中丙酮酸对ALT测定结果的影响及其消除方法[J]. 现代检验医学杂志, 1999, 14(4): 24-26 |
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| 作者姓名： | 王春新 杨荣伟 |
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| 作者单位： | [1]无锡市第一人民医院检验科 [2]浙江医科大学第一附属医院检验科 |
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| 摘 要： | 实验观察了试剂及血清中丙酮酸浓度对单试剂动力学法及以丙氨酸为第二试剂的双试剂法测定ＡＬＴ的影响。单试剂法测定,以血清（或试剂）中丙酮酸浓度对ＡＬＴ活性回归,回归系数ｂ与ｏ的检验,Ｐ均小于０．００１,说明ＡＬＴ表观活性受血清（或试剂）中丙酮酸浓度影响;用丙氨酸为第二试剂的双试剂法测定,以血清（或试剂）中丙酮酸浓度对ＡＬＴ活性回归,回归系数ｂ与ｏ的检验,Ｐ均大于０．０５,说明ＡＬＴ表观活性不受血清（或
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| 关 键 词： | 丙酮酸 ALT 双试剂法 |
The Effect of Pyruvate on ALT Assay with Its Resolving Method |
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| WANG Chun-Xin, YANG Rong-Wei. The Effect of Pyruvate on ALT Assay with Its Resolving Method[J]. Journal of Modern Laboratory Medicine, 1999, 14(4): 24-26 |
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| Authors: | WANG Chun-Xin YANG Rong-Wei |
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| Abstract: | We investigated the effect of pyruvate concentration in serum(or solution) on alanine transaminase (ALT) activity by one step and two step procedures. Regression of serum(or solution) pyruvate against ALT ac-tivity and test of coefficient b with 0 were made in both assays. It indicated that ALT activity was influenced by serum (or solution) pyruvate in one-step procedure (P<0. 001) while not in two-step assay which alanine was added afterward (P>0. 05). Same conclusion was also made from match test of ALT activity before and after pyruvate added(P>0. 05) |
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| Keywords: | Pyruvate ALT Two-step-assay |
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