| 蛋白激酶C对高糖作用下腹膜间皮细胞葡萄糖转运蛋白1调节作用 |
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| 引用本文： | 丁红,马健飞,樊怡. 蛋白激酶C对高糖作用下腹膜间皮细胞葡萄糖转运蛋白1调节作用[J]. 中国血液净化, 2010, 9(1): 37-40 |
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| 作者姓名： | 丁红 马健飞 樊怡 |
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| 作者单位： | 中国医科大学附属第一医院肾内科,沈阳,110001 |
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| 摘 要： | 目的观察蛋白激酶C(PKC)对高糖作用下体外培养的大鼠腹膜间皮细胞(PMCs)胞膜上葡萄糖转运蛋白1(GLUT1)表达的调节,以探讨长期腹膜透析时如何避免腹膜损伤、改善腹膜透析疗效。方法胰酶消化法提取雄性Wistar大鼠的PMCs细胞进行培养,分别加入不同浓度葡萄糖及PKC抑制剂Go6976,间接免疫荧光法检测PMCs上GLUT1表达,流式细胞仪检测作用前后细胞膜上GLUT1量的改变,生化分析仪检测培养液内葡萄糖浓度的变化。结果高糖可增加细胞膜上GLUT1的表达,同时伴有PMCs对葡萄糖的转运增加(P0.05)。Go6976明显抑制了高糖对GLUT1的诱导,且葡萄糖的转运亦下降。结论葡萄糖以浓度依赖方式上调PMCs细胞膜上GLUT1的蛋白表达,同时伴有PMCs对葡萄糖转运的增加。PKC通路在其中发挥重要作用,应用PKC抑制剂可拮抗高糖对GLUT1表达的诱导作用。
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| 关 键 词： | 腹膜间皮细胞 葡萄糖转运蛋白1 蛋白激酶C |
PKC's effect on glucose transporter 1 with high glucose in peritoneal mesothelial cells |
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| DING Hong,MA Jian-fei,FAN Yi. PKC's effect on glucose transporter 1 with high glucose in peritoneal mesothelial cells[J]. Chinese Journal of Blood Purification, 2010, 9(1): 37-40 |
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| Authors: | DING Hong MA Jian-fei FAN Yi |
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| Affiliation: | DING Hong,MA Jian-fei,FAN Yi.Department of Nephrology,The First affiliated Hospital,China Medical University,Shenyang 110001,China |
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| Abstract: | Objective In order to avoid peritomum injury and to improve the efficiency of peritoneal dialysis during the dialysis,We investigated the regulating effect of protein kinase C(PKC) on Peritoneal Mesothelial Cells(PMCs) of the plasma membrane cultivated in high concentrations of glucoses.Methods PMCs that had been harvested from male Wistar rats by Enzymatic disaggregation method was cultivated in different concentrations glucoses.PKC inhibitor G6976 was applied to obstruct the functions of PKC.Indirect immu... |
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| Keywords: | peritoneal mesothelial cell glucose transporter 1 protein kinase C |
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