Normothermic liver ischemia in rats: xanthine oxidase is not the main source of oxygen free radicals |
| |
Authors: | Witold Karwinski Bjørn Bolann Rune Ulvik Mikael Farstad Odd Søreide |
| |
Institution: | 1. Department of Surgery, Haukeland Hospital, University of Bergen, N-5021, Bergen, Norway 2. Department of Clinical Biochemistry, Haukeland Hospital, University of Bergen, N-5021, Bergen, Norway
|
| |
Abstract: | We studied the effect of allopurinol (ALL) on the activity of xanthine dehydrogenase (XDH), xanthine oxidase (XOX), superoxide
dismutase (SOD), and catalase (CAT) in rat liver during ischemia followed by 60 min of reperfusion. We induced 60-min ischemia
in the median and left lobes by clamping the hepatic artery and portal branches. The percentage XOX relative to total oxidase
activity increased significantly in the control group, from 10% during the stabilization period to 18% after 60 min of reperfusion.
The XDH activity decreased during reperfusion. Activity of both XDH and XOX was almost completely blocked by ALL. The activity
of SOD and CAT did not differ significantly between the ALL group and controls after 60 min of reperfusion. ALL treatment
did not affect liver injury parameters, as concentrations of lactate dehydrogenase (LDH) and alanine transferase (ALT) increased
in plasma after ischemia, both in controls and in the ALL-treated group. We concluded that ischemia promotes conversion of
XDH to XOX during reperfusion. XOX may not be the main source of free radical production, since intracellular scavengers (SOD
and CAT) did not differ significantly between controls and the ALL-treated group, despite the fact that ALL blocked XOX activity
completely. |
| |
Keywords: | Liver Ischemia Allopurinol Xanthine oxidase Xanthine dehydrogenase Superoxide dismutase Catalase |
本文献已被 SpringerLink 等数据库收录! |
|