| Expression of CD28 and CTLA4 on T Cells in Bone Morrow of Immune-mediated Aplastic Anemia Mice |
| |
| 作者姓名： | 刘振芳 孙汉英 刘文励 罗小华 何莉 徐慧珍 |
| |
| 作者单位： | Department of Hematology, Tongji Hospital. Tongji Medical College, Haazhong University of Science and Technology,Wuhan 430030, China |
| |
| 基金项目： | This project was supported by a grant from National Natural Sciences Foundation of China （No. 39870926）. |
| |
| 摘 要： | Summary： To investigate the expression and significance of CD28 and CTLA4 on T cells in bone marrow of aplastic anemia （AA） mice, in vitro bone marrow mononuclear cells （BMMNCs） were activated through being incubated with PHA （15 μg/mL）. The expression of CD28 and CTLA4 on T cells incubated with or without PHA was detected by two-color flow cytometry. The expression of CD28 and CTLA4 was significantly increased after PHA stimulation. In the AA mice. the expression of CD28 with or without PHA stimulation was both higher than that in the normal mice （both P〈0.01）, but the expression of CTLA4 with or without PHA stimulation showed no significant difference in comparison to that in the normal mice （both P〉0.05）. In the AA mice, there were more activation and activated potential of T cells than the normal, and the abnormal expression of CD28 and CTLA4 may participate in immunological disorder mediated by T cells.
|
| 关 键 词： | CD28 CTLA4 T细胞 基因表达 骨髓 免疫调节 小鼠 动物实验 贫血 |
| 收稿时间： | 2005-03-15 |
Expression of CD28 and CTLA4 on T cells in bone morrow of immune-mediated aplastic anemia mice |
| |
| Liu Zhenfang,Sun Hanying,Liu Wenli,Luo Xiaohua,He Li,Xu Huizhen.Expression of CD28 and CTLA4 on T Cells in Bone Morrow of Immune-mediated Aplastic Anemia Mice[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(5):508-509. |
| |
| Authors: | Liu Zhenfang Sun Hanying Liu Wenli Luo Xiaohua He Li Xu Huizhen |
| |
| Institution: | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China |
| |
| Abstract: | Summary To investigate the expression and significance of CD28 and CTLA4 on T cells in bone marrow of aplastic anemia (AA) mice,in vitro bone marrow mononuclear cells (BMMNCs) were activated through being incubated with PHA (15 μg/mL). The expression of CD28
and CTLA4 on T cells incubated with or without PHA was detected by two-color flow cytometry. The expression of CD28 and CTLA4
was significantly increased after PHA stimulation. In the AA mice, the expression of CD28 with or without PHA stimulation
was both higher than that in the normal mice (bothP<0.01), but the expression of CTLA4 with or without PHA stimulation showed no significant difference in comparison to that
in the normal mice (bothP>0.05). In the AA mice, there were more activation and activated potential of T cells than the normal, and the abnormal expression
of CD28 and CTLA4 may participate in immunological disorder mediated by T cells.
LIU Zhenfang, female, born in 1976, Resident
This project was supported by a grant from National Natural Sciences Foundation of China (No. 39870926). |
| |
| Keywords: | aplastic anemia lymphocyte antigen cell surface CD28 CTLA4 |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
