p27蛋白表达对肾硬化大鼠肾小球细胞增殖的影响

目的：研究p27蛋白表达对肾硬化大鼠肾小球细胞增殖的影响。方法：建立阿霉素肾硬化大鼠模型，用RT-PCR方法检测增殖细胞核抗原(PCNA) mRNA的表达，免疫组化检测肾小球内PCNA和p27的表达。结果：疾病组PCNA mRNA表达明显增强，增殖指数进行性升高。对照组p27蛋白有一定水平表达，而疾病组其表达进行性下降；p27蛋白表达与增殖指数和PCNA mRNA表达呈负相关。结论：肾硬化过程中肾小球细胞增殖呈进行性增加，p27表达下调参与调控肾小球细胞的增殖。

The effect of p27 expression on glomerular cell proliferation in experimental glomerulosclerosis

Objective: To study the effect of p27 expression on glomerular cell proliferation in experimental glomerulosclerosis. Methods:The rat glomerulosclerosis model was induced by Adriamycin. Proliferation was determined by semi-quantitative RT-PCR assay and immunohistochemistry on proliferation cell nuclear antigen(PCNA). The glomerular cell p27 protein expression was evaluated by immunohistochemistry at th study point. Results:Semi-quantitative RT-PCR revealed increased PCNA mRNA expression in the experimental glomeruli, whereas no PCNA mRNA expressed in the control glomeruli, which coincided with the results of proliferation indices. p27 decreased progressively There was significantly negative correlation between p27 and PCNA mRNA expression(r=0.8043,P<0.05). Conclusions:Glomerular cell proliferation increased progressively with the development of glomerulosclerosis, the decreased p27 expression is likely to participate in the regulation of glomeular cell proliferation.