| 家兔实验性心肌缺血肌动蛋白免疫组化定量研究 |
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| 引用本文： | 董建国,宫丽平,张秀梅,闫广泽,孙慧宽,高林桃. 家兔实验性心肌缺血肌动蛋白免疫组化定量研究[J]. 中国体视学与图像分析, 1999, 0(3) |
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| 作者姓名： | 董建国 宫丽平 张秀梅 闫广泽 孙慧宽 高林桃 |
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| 作者单位： | 内蒙古医学院病理解剖学教研室!呼和浩特010059(董建国,宫丽平,孙慧宽),内蒙古医学院第一附院外科!呼和浩特010059(张秀梅),内蒙古医学院第一附院核医学科!呼和浩特010059(闫广泽),内蒙古医学院病理解剖学教研室!呼和浩特01(高林桃) |
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| 摘 要： | 本文通过建立家兔心肌急性缺血的动物模型 ,应用免疫组织化学方法和图像分析技术 ,结合HE和PAS染色对心肌细胞肌动蛋白在心肌缺血时的早期改变进行了观察和测定。当心肌缺血 30min时 ,肌动蛋白发生了缺失 ,因此可以作为心肌早期缺血的指标。同时观察到在心肌缺血 1h内 ,肌动蛋白的阳性面积和强度随缺血时间的延长而显著降低 ,可较准确客观地判定心肌缺血时间。
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| 关 键 词： | 实验性心肌缺血 免疫组织化学 肌动蛋白 图像分析 |
Immunohistochemical and image analysis on quantitative study of actin expression during experimental myocardial ischemia in rabbits |
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| Dong Jinguo Gong Liping Zhang Xiumei. Immunohistochemical and image analysis on quantitative study of actin expression during experimental myocardial ischemia in rabbits[J]. Chinese Journal of Stereology and Image Analysis, 1999, 0(3) |
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| Authors: | Dong Jinguo Gong Liping Zhang Xiumei |
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| Abstract: | The early histochemical changes of actin in ischemic myocardium following coronary artery occlusion in 18 rabbits were studied by histochemical, immunohistochemical and image analysis techniques. The resulrs shoued that the amount and the IRS of actin were significantly diminished 30min after ischemia (P<0.05). 60min after occlusion, the intensities and areas of actin were progressively markedly reduced. It is suggested the immunohistochemical changes of action may be used as an early criterion for acute myocardial ischemia, and it provides an accurate method for detemining the stage of early myocardial ischemia. |
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| Keywords: | experimental myocardial ischemia immunohistochemistry actin image morphometry. |
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