| Bcl-XL siRNA抑制人结肠癌细胞生长的研究 |
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| 引用本文： | 胡静姿,李玉莉,刘惠敏,孙志勇,杨志慧. Bcl-XL siRNA抑制人结肠癌细胞生长的研究[J]. 中国肿瘤生物治疗杂志, 2006, 13(1): 42-44 |
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| 作者姓名： | 胡静姿 李玉莉 刘惠敏 孙志勇 杨志慧 |
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| 作者单位： | 第二军医大学长征医院病理科,上海,200003 |
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| 摘 要： | 目的:探讨应用siRNA技术下调Bcl-XL对人结肠癌细胞生长的抑制作用。方法:采用人结肠癌细胞株DLD1, 利用人工合成的Bcl-XL靶向小分子干扰RNA(siRNA)转染DLD1细胞,通过Western blot法检测转染细胞Bcl-XL蛋白的表达,流式细胞仪检测处理后细胞的凋亡情况,锥虫蓝染色法计数存活细胞。结果:与空白组和对照组相比,转染Bcl-XL siR- NA组的Bcl-XL蛋白表达显著性下降,凋亡细胞的数量显著增加(P<0．05),细胞存活率显著降低(P<0．05)。结论:Bcl-XL siRNA能显著降低人结肠癌细胞的Bcl-XL蛋白表达,有效抑制细胞的生长,为结肠癌的治疗提供了一种新的治疗策略。
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| 关 键 词： | 结肠癌 |
| 文章编号： | 1007-385X（2006）01-0042-03 |
| 收稿时间： | 2005-10-15 |
| 修稿时间： | 2005-11-20 |
Bcl-XL siRNA in inhibition of human colon cancer cells growth |
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| HU Jing-zi,LI Yu-li,LIU Hui-min,SUN Zhi-yong and YANG Zhi-hui. Bcl-XL siRNA in inhibition of human colon cancer cells growth[J]. Chinses Journal of Cancer Biotherapy, 2006, 13(1): 42-44 |
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| Authors: | HU Jing-zi LI Yu-li LIU Hui-min SUN Zhi-yong YANG Zhi-hui |
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| Affiliation: | Department of Pathology, Changzheng Hospital, Second Military Medical University,Shanghai 200003 ,China;Department of Pathology, Changzheng Hospital, Second Military Medical University,Shanghai 200003 ,China;Department of Pathology, Changzheng Hospital, Second Military Medical University,Shanghai 200003 ,China;Department of Pathology, Changzheng Hospital, Second Military Medical University,Shanghai 200003 ,China;Department of Pathology, Changzheng Hospital, Second Military Medical University,Shanghai 200003 ,China |
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| Abstract: | Objective: To study the inhibitory effect of a double-strand small interfering RNA targeting Bcl-XL (Bcl-XL siRNA) in human colon cancer cells. Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA. The apoptotic ratio was significantly higher in Bcl-XL siRNA treated group than those in control (P<0.05) , whereas the survival rate of cells was significantly decreased in Bcl-XL siRNA group(P <0. 05). Conclusion: Bcl-XL siRNA can effectively knock down the Bcl-XL protein and inhibit the growth of human colon cancer cells, which might be a new way for colon cancer treatment. |
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| Keywords: | Bcl-XL siRNA |
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