胆汁细菌培养与药敏分析

目的：了解胆汁中病原菌菌群分布情况，分析胆汁中细菌药物敏感性。方法：应用BACTEK9120培养系统和VITEK32全自动细菌鉴定及药敏分析系统对81例胆汁标本进行细菌鉴定和药敏分析。结果：胆汁细菌培养阳性率为88．9％，主要菌种有大肠埃希菌、铜绿假单胞菌、肠球菌，肠球菌比率较高；需氧茵中革兰氏阴性菌对亚胺培南、哌拉西林／他唑巴坦、头孢他啶的敏感性较好．肠球菌只对左氧氟沙星、氨苄西林敏感性较好。结论：胆汁细菌培养阳性率很高，有一定的临床价值。

Cultivating bacteria in bile and detecting their sensitivity to antibiotics

Objective: To investigate the distribution of pathogenic microorganisms in bile and to study the sensitivity to antibiotic drugs of the microorganisms. Method: To cultivate the microorganisms in 81 bile samples and study the sensitivity to antibiotic drugs of the microorganisms with BACTEK9120 culturing system and automatic VITEK32 bacterial culturing and antibiotic drug sensitivity studying system. Result: The positive rate of bile bacteria was 88.9%, the major general of bacteria were E.coli, Pseudomonas aeruginosa and Enterococcus. In aerobes, gram negative bacteria was more sensitive to Imipenem , Tazobactam/piperacillin, Ceftazibime. Enterococcus was only sensitive to Levuofloxacin, Ampicillin . Conclusion: The positive rate of bacteria in bile was very high, which was useful for clinic.