HO-1参与葛根素对抗高糖诱导的大鼠血管舒张功能下降

目的： 研究葛根素对抗急性高糖刺激引起的血管舒张功能的下降，并分析血红素加氧酶（HO-1）在其中的作用。方法: 采用血管环灌流装置，观察大鼠胸主动脉环的舒张效应。结果: ①与空白对照组（含11 mmol/L葡萄糖）相比，经44 mmol/L葡萄糖（高糖）孵育血管2 h后，主动脉环对ACh引起的内皮依赖性血管舒张反应下降。②葛根素（10-10-10-8 mol/L）与高糖联合孵育，可剂量依赖性地改善高糖诱导的血管ACh舒张反应的下降。③葛根素孵育血管后可引起血管HO-1活性增高；用ZnPPIX抑制HO-1的活性后，葛根素抗高糖损伤的作用被取消。结论: 葛根素可以对抗高糖引起的血管舒张功能的下降，其机制可能是通过诱导HO-1而实现的。

Puerarin protects high glucose-induced injury of vascular relaxation by activation of heme oxygenase-1

AIM: To investigate the effect of puerarin on acute high glucose-induced attenuation of ACh relaxation in isolated rat aortic rings, and to elucidate its underlying mechanism. METHODS: The thoracic aortic rings with endothelium of male Sprague-Dawley rats were mounted on a bath system. Isometric relaxation of aortic rings was measured. RESULTS: ① After incubation with high concentration of glucose (44 mmol/L), the vascular relaxation responses to ACh decreased. ② After coincubation with puerarin (10-10-10-8 mol/L) and high glucose, the high glucose-induced attenuation of ACh relaxation responses of artery was partly inhibited in a dose-dependent manner. ③ After incubation with puerarin for 2 h, the HO-1 activity of thoracic aorta increased. ZnPPIX (an inhibitor of heme oxygenase-1) abrogated the protective effect of puerarin. CONCLUSION: Puerarin prevents the acute high glucose-induced attenuation of endothelium-dependent relaxation in aortic rings. The mechanism might be involved in the activation of heme oxygenase-1.