白金胶囊对CUMS诱导的抑郁症大鼠模型行为学及脑内单胺类神经递质的影响

目的：观察白金胶囊对慢性不可预知温和应激（ CUMS）致抑郁症大鼠模型行为学及脑内单胺类神经递质的影响。方法采用慢性不可预知温和应激结合孤养的方法建立抑郁症大鼠模型，造模7周后，采用白金胶囊（12.6 g／kg、4.2 g／kg、1.4 g／kg）和盐酸氟西汀（3.5 mg／kg）连续灌胃4周进行治疗，给药期间继续实施应激方案，共计11周，另设正常对照组，常规饲养。监测糖水偏嗜度、以及动物在旷场中的水平运动、垂直运动等行为学指标；实验终点，采用高效液相-电化学法测定大鼠脑皮层、海马部位单胺类神经递质（5-HT、DA、NE及其代谢产物）的水平。结果与正常对照组比较，模型大鼠造模7周后，体重、糖水消耗百分比、旷场水平运动和垂直活动明显降低（ P＜0.05，P＜0.01）；与模型组比较，白金胶囊连续给药4周后，各剂量可显著增加糖水消耗百分比，旷场水平运动距离和垂直运动次数（ P＜0.05，P＜0.01），改善抑郁动物行为学；可升高抑郁模型脑皮层中5-HT、DA以及NE的含量（ P＜0.05）。结论白金胶囊可改善CUMS致抑郁症大鼠模型的行为学异常，作用机制可能与增加脑皮层中单胺类神经递质含量有关。

The Pharmacodynamic Research of Baijin Capsule on Depression in

Objective To evaluate the effects of baijin capsule on behavioral changes and monoamine neurotransmitters concentration in chronic unpredictable mild stress (CUMS)depression rat model. Methods The depression rat model was induced by11-week chronic unpredictable mild stress combining with solitary. After the model were established, rats were given the decoction of baijin capsule (12.6 g/kg, 4.2 g/kg, 1.4 g/kg) or fluoxetine hydrochloride (3.5 mg/kg) by intragastricfor 4 weeks. During the experiment period, sucrose consumption and open-field experiment were conducted to monitor the behavior of rats, such as sucrose consumption percentage, horizontal motion, and vertical motion. At the end of the experiment, the levels of the monoamine neurotransmitters in the cerebral cortex and hippocampus were analyzed by method of high performance liquid chromatography-electrochemistry. Results Compared with the normal group, the weight, horizontal displacement distance, vertical movement times, and sucrose consumption percentage of rats in model group decreased significantly after stimulated with CUMS and solitary for 7 weeks (P<0.05,P<0.01).Compared with model group, consecutively administrated for 4 weeks, horizontal displacement distance, vertical movement times, and the percentage in sugar water consumption significantly increased with the treatment of baijin capsule (P<0.05, P<0.01). Meanwhile, the content of norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamine (5-HT) in the cortex were significantly increased in rats of the baijin capsule (P<0.05). Conclusions The results indicated that baijin capsule improved the behavioral disturbances in depression rat model, which were related to enhancement of the concentration of monoamine neurotransmitters in the cortex.