RNA sequencing steps toward the first line |
| |
| Authors: | Theresa A Boyle MD PhD Aaron D Bossler MD PhD |
| |
| Institution: | Department of Pathology, Moffitt Cancer Center, Tampa, Florida, USA |
| |
| Abstract: | - DNA is the sequence that codes for proteins.
- Messenger RNA is transcribed from the DNA sequence of genes and translated into protein.
- It can be difficult to predict how a change in the DNA sequence will affect messenger RNA and protein quantity and quality.
- DNA translocation changes can cause the joining of sequences from two different genes or different parts of the same gene.
- DNA sequencing is often used clinically to predict how DNA changes might affect proteins.
- Alternatively, RNA sequencing can be used as a more direct measure of the effect of DNA changes on the protein products.
- This sequencing is important for identifying changes in cancer that may indicate response to targeted therapy, prognosis, or diagnosis.
|
| |
| Keywords: | biomarkers fusion protein next-generation sequencing (NGS) nonsmall cell lung cancer RNA sequencing splice variant targeted therapy translocation |
|
|
