Telomerase activity in well-differentiated papillary thyroid carcinoma corrrelates with advanced clinical stage of the disease

Clinical relevance and stage correlation of telomerase activity in well-differentiated papillary thyroid carcinoma (WD PTC) has not been well determined, as its reported activity could be due to the analysis of tumors with lymphocytic infiltrates or aggressive variants of papillary carcinomas. We conducted a prospective study of telomerase activity in WD PTC without inflammatory infiltrates and correlated it with clinical stage. Fifty WD PTCs were analyzed for telomerase activity by PCR-based TRAP (telomeric repeat amplification protocol) assay. Results were correlated with stage and other clinicopathologic variables. Twenty-one (42%) WD PTCs demonstrated telomerase activity. The enzyme was detected more frequently in stage III/IVa WD PTCs (p=0.02) and in tumors with extra thyroidal extension (p=0.04). The risk of presenting advanced disease (stage III/IVa) and extrathyridal growth was significantly increased in telomerase-positive tumors (p=0.01; odds ratio OR] 4.4 95%Cl 1.3–14.7]) and (p=0.04; OR 3.6 95%Cl 1.1–11.7]), respectively. Also, a correlation was found between telomerase activity and age. There was no correlation of telomerase activity with gender, histologic variant, tumor size, or cervical lymph node metastasis. Telomerase activity was observed in 42% of WD PTC and was detected more frequently in AJCC TNM stage III/IVa cases. This finding suggests that telomerase deregulation could be involved in tumor progression.