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| 可对细胞施加双向应变的体外培养系统 | |
| 引用本文： | 陈孟诗,李良,邓力,黄华,赖胜祥.可对细胞施加双向应变的体外培养系统[J].生物医学工程学杂志,2003,20(3):430-433. |
| 作者姓名： | 陈孟诗 李良 邓力 黄华 赖胜祥 |
| 作者单位： | 1. 四川大学,力学系,成都,610065 2. 四川大学,生物医学工程研究室,成都,610064 3. 四川大学,药学院,成都,610064 4. 四川大学,通讯工程系,成都,610065 |
| 基金项目： | 国家自然科学基金资助项目 (10 0 72 0 5 ,3 9870 2 0 1) |
| 摘 要： | 为考察体外培养细胞在动应变作用下的响应，参考Winston等人的方法，我们制作了一套细胞体外培养系统，培养皿用有机玻璃作成，培养皿底为硅橡胶膜，下面有一封闭压力腔，通过对硅橡胶管和挤压轮间隙的调整，压力腔内的液压使硅橡胶膜产生设计应变，其应变范围可从0～4％调整，改变步进电机的转动方式，加载频率可从0．1～5Hz变化。 |
| 关 键 词： | 双向应变 体外细胞培养 细胞加载培养系统 硅橡胶薄膜 有限元计算 |
A System Applying Cyclic Biaxial Mechanical Strain to Cultured Cells |
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| Mengshi Chen,Liang Li,Li Deng,Hua Huang,Shengxiang Lai.A System Applying Cyclic Biaxial Mechanical Strain to Cultured Cells[J].Journal of Biomedical Engineering,2003,20(3):430-433. | |
| Authors: | Mengshi Chen Liang Li Li Deng Hua Huang Shengxiang Lai |
| Institution: | Department of Mechanical Engineering, Sichuan University, Chengdu 610065. |
| Abstract: | To study the response of cultured cells to the cyclical biaxial biomechanical strain ,a system was made according to the principle of Winston. The system was made of culture dish,pressure cavity and step motor. The culture dishes were made of polymethyl methacrylate and the bottom of the culture dishes were made of silicone rubber membrane. When the diameter of the silicone rubber tube and the distance between rollers and curvilinear plate changed ,the pressure in the pressure cavity changed and the strain of the membrane changed from 0 to 4%. When the roll of the step motor changed, the frequency of the strain changed from 0.1 to 5 Hz. |
| Keywords: | Strain Cell Cell culture in vitro |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |