增生性玻璃体视网膜病变增生膜内炎性细胞因子mRNA表达

目的 检测炎前因子mRNA在增生性玻璃体视网膜病变(proliferative vitreoretinopathy, PVR)中的表达情况，证实其在PVR发病中的作用。 方法 收集14例不同分级的PVR患者玻璃体切割术中所取出的增生膜，石蜡包埋切片，用生物素标记的寡核苷酸探针进行原位杂交，检测IL-1β、IL-6、IL-8和TNF-αmRNA的表达，两例角膜移植后的供体眼作为正常对照。 结果 共有5例样本有阳性表达。3例增生膜中IL-1βmRNA表达，3例表达了IL-6 mRNA，1例表达了IL-8 mRNA，3例表达TNF-αmRNA。其中1例既表达了IL-1β又表达了IL-6 mRNA，1例IL-1β、IL-8和TNF-αmRNA三者均表达，2例有IL-6和TNF-αmRNA两者表达。正常视网膜未检测到炎前因子mRNA的表达。 结论 IL-1β、IL-6、IL-8和TNF-α参予了PVR增生膜的形成过程。 (中华眼底病杂志, 2002, 18: 286-288)

The mRNA expression of proinflammatory factors in periretinal membrane of proliferative vitreoretinopathy

Objective To observe the expression of proinflammatory factors messenger RNA(mRNA) in periretinal membrane of proliferative vitreoretinopathy. Methods Fourteen specimens of periretinal membrane were collected during vitrectomy, and they were made into paraffin sections.The presence of mRNA coding for IL-1,IL-6,IL-8 and TNF alpha was observed by in situ hybridization(ISH) with biotin labeled oligonuclotide probes respectively.The eyeball after corneal grafting was made as normal control. Results No expression of proinflammatory factors mRNA was found in normal human retina.Positive staining was present in 5 specimens.In these specimens, IL-1βmRNA was found in 3 specimens and TNFαmRNA was found in 3 specimens,there is 1 specimen expressed IL-8 mRNA and 3 specimens expressed IL-6 mRNA.In these positive specimens, one contained cells expressing mRNA for IL-1βbeta and IL-6, and one exhibited cells expressing mRNA for IL-1β、IL-8 and TNFα,two membranes possessed positive cells for IL-6 and TNFαmRNA, one membrane contained IL-1βmRNA positive cells only. Conclusion These findings suggest that these cytokines may be locally produced by cells infiltrating epiretinal membranes. The expression of IL-1β, IL-6, IL-8 and TNFαmRNA within retinal membranes provides further evidence of a pathogenic role of these cytokines in proliferative vitreoretinopathy. (Chin J Ocul Fundus Dis, 2002, 18: 286-288)