肥大细胞的浸润对胃癌生长转移的影响

目的 探讨肥大细胞的浸润与胃癌生长﹑转移的关系。方法 采用阿尔新兰-沙红染色与显微图像分析系统相结合的技术,对74例胃癌组织内的肥大细胞进行显色﹑分类及定量计数。结果 胃癌组织内的肥大细胞主要分布于癌旁交界区;以成熟型为主,功能活跃,释放浆内颗粒。癌旁交界区肥大细胞的数量显著多于癌内间质区(t=9.11,P<0.01),两者呈正相关性(r=0.303,P<0.01)。肥大细胞的数量与胃癌浸润深度及淋巴结转移数目呈显著的负相关性。结论 癌间质内肥大细胞的浸润具有抑制胃癌生长﹑转移的作用。可能与成熟型肥大细胞释放肝素及其他生物活性物质有关。癌旁交界区肥大细胞的数目可作为判断预后的指标之一。

Effects of mast cell infiltration on the development and metastasis of gastric carcinoma

Objective To investigate the relationship between mast cell infiltration and the development and metastasis of gastric carcinoma.Methods Alcian blue-Safranin O staining and image analysis system were employed to observe,classify and quantify the mast cells in 74 gastric carcinoma specimens.Results Mast cells resided predominantly in the marginal area of the tumor and most of them were mature type,active and capable of discharging granules into the cytoplasm.The mast cells in the marginal area of the tumor were significantly more numerous than those in the matrix of the tumor (P<0.01) and there was a positive correlation between them (r=0.303,P<0.01).The density of the mast cells was inversely correlated to the depth of tumor invasion and the number of lymph node metastasis.Conclusions Mast cell infiltration in the matrix of the tumor plays an important role in preventing the invasion and metastasis of gastric cancinoma possibly by release of heparin and other bioactive substances.Mast cell quantity in the marginal area of the tumor may serve as an indicator for prognostic assessment of gastric carcinoma.