心绞痛患者血清hsCRP、MIF及IL-10水平与临床分型研究

目的检测稳定型心绞痛患者及不稳定型心绞痛患者外周血血清高敏C反应蛋白（hsCRP）、巨噬细胞移动抑制因子（MIF）、白介素10（IL-10）水平,了解其变化与患者临床分型之间的关系。方法共收入43例稳定型心绞痛患者和50例不稳定型心绞痛患者,同时收入40例健康志愿者作为对照组,以ELISA法检测其外周血血清中hsCRP、MIF、IL-10水平并进行统计分析。结果不稳定型心绞痛患者血清hsCRP水平（12.53±2.89）mg/L高于对照组（3.46±1.02）mg/L及稳定型心绞痛患者（4.51±0.98）mg/L,差异有统计学意义;不稳定型心绞痛患者MIF水平（30.94±5.85）μg/L高于对照组（8.31±1.38）μg/L及稳定型心绞痛患者（9.85±1.44）μg/L,差异有统计学意义;稳定型心绞痛患者及对照组hsCRP及MIF水平比较差异无统计学意义。不稳定型心绞痛患者及稳定型心绞痛患者血清IL-10水平分别为（19.44±2.11）ng/L和（20.19±2.04）ng/L,与对照组（14.89±2.49）ng/L比较差异有统计学意义。结论稳定型心绞痛与不稳定型心绞痛患者的血清炎症反应与抗炎反应生物标志物存在不同的表达情况,提示心绞痛患者体内炎症反应与抗炎反应平衡与患者病情进展之间存在一定的关系。

Detection of high-sensitivity C-reactive protein,macrophage migration inhibitory factor and interleukin-10 in patients with angina pectoris

Objective To explore the relationship between angina pectoris clinical subtype and biomarkers by detecting the level of serum high-sensitivity C-reactive protein（hsCRP）,macrophage migration inhibitory factor（MIF）and interleukin-10 （IL-10） in patients with stable angina pectoris and unstable angina pectoris. Methods 43 cases of stable angina pectoris patients and 50 cases of unstable angina pectoris patients were enrolled into the study. 40 cases of healthy volunteers were enrolled into the controlled group.The serum level of hsCRP,MIF and IL-10 was detected by ELISA. The data were then analyzed by One-way ANOVA. Results The serum level of hsCRP （12.53±2.89）mg/L in unstable angina pectoris patients was significantly higher than the controlled group （3.46±1.02）mg/L and stable angina pectoris patients （4.51±0.98）mg/L （P0.05）. The serum level of MIF （30.94±5.85）μg/L in unstable angina pectoris patients was significantly higher than the controlled group （8.31±1.38）μg/L and stable angina pectoris patients（9.85±1.44）μg/L（P0.05）. There was no significant difference in the level of hsCRP and MIF between the stable angina pectoris and controlled group. The serum level of IL-10 in the unstable and stable angina pectoris group was （19.44±2.11）ng/L and（20.19±2.04）ng/L, respectively. The value was significantly higher than the controlled group （14.89±2.49） ng/L （P0.05）. Conclusion A distinct pattern of inflammatory and anti-inflammatory biomarkers in patients with stable and unstable angina pectoris was observed. The balance of inflammatory and anti-inflammatory response may be associated with the disease progress.