高危型HPV感染与食管鳞癌组织中HLA-G表达的相关性研究

目的研究人乳头瘤状病毒（HPV）在新乡地区食管鳞癌、癌旁组织中的感染情况和人类白细胞抗原系统G（HLA-G）表达的关系。方法采用基因芯片技术检测114例食管鳞癌组织及癌旁食管正常组织中HPV亚型感染情况,免疫组化SP法检测HLA-G的表达。结果 114例食管鳞癌组织及癌旁食管正常组织中HPV感染阳性率分别为63.2%和6.0%,HLA-G的检出率分别为51.8%、0%,差异均有统计学意义（P〈0.05）;食管鳞癌存在HPV16、18和52三种亚型感染,其中HPV16感染率最高,HPV52感染率最低。结论新乡地区食管鳞癌组织以HPV16、18和52亚型感染为主;HPV感染和HLA-G蛋白表达与食管鳞癌分化程度正相关;HPV感染食管鳞癌组织与HLA-G蛋白表达存在一定的相关性,可能提示与食管鳞癌发生有较密切关系。

Correlation of high risk HPV infection and HLA-G expression in esophageal carcinomas

Objective The aim of this study was to investigate the relationship between different human papillomavirus（HPV） genotypes and the expression of HLA-G in esophageal carcinomas.Methods A total of 114 cases of esophageal carcinomas and 50 cases of esophagus were assayed for expression of HLA-G by immunohistochemistry.HPV typing was carried out by gene chip.Results Overall,HPV and HLA-G prevalence were 63.2%,6.0% and 51.8%,0 among the esophageal carcinomas and esophagus in this study,respectively.HPV-16,HPV-18,HPV-52 were detected 34.2%,16.7% and 12.3% in the tumors,respectively.The expression of HLA-G was not related to HPV genotype.However,in the HPV-18 positive esophageal carcinomas,expression of HLA-G was significantly increased（P0.05）.Conclusion HPV-16,HPV-18 and HPV52 genotypes can be found in ESCC specimens collected from Xinxiang regions.Up-regulation of HLA-G was strongly associated with HPV-18 positive esophageal carcinomas.It supports the hypothesis that HLA-G proteins may play an important role in the inactivation and degradation of carcinogenesis of HPV（especially HPV18 genotype） positive esophageal neoplasia.