白介素32在胃癌细胞中的表达及意义

目的观察白介素32（IL-32）在胃癌细胞株中的表达情况,探讨其在胃癌发生、发展中的意义。方法采用逆转录PCR、real-time PCR及Western blot法分别从基因和蛋白水平上检测IL-32在正常胃上皮细胞GES-1以及胃癌细胞株MKN-45、KATO中的表达情况。结果逆转录PCR及real-time PCR结果均显示胃癌细胞IL-32 mRNA表达水平明显高于正常胃上皮细胞,逆转录PCR灰度比值（目的 /内参）为：GES-1（0.34±0.09）、MKN-45（0.79±0.11）、KATO（0.90±0.17）,差异有统计学意义（P〈0.05）。real-time PCR Ct值显示：MKN-45的IL-32基因表达量是GES-1的（5.34±1.09）倍,KATO是GES-1的（4.07±1.69）倍,差异均有统计学意义（P〈0.05）。Western blot结果显示IL-32在蛋白表达水平的结果与前一致,灰度比值（目的 /内参）为：GES-1（0.30±0.10）、MKN-45（0.92±0.32）、KATO（0.86±0.15）,差异有统计学意义（P〈0.05）。结论IL-32在胃癌细胞株中呈高表达,推测其可能在胃癌的发生、发展中起一定作用。

Expression of IL-32 and its roles in the human gastric cancer cell lines

Objective To observe the interleukin-32 expression in gastric cancer cell lines,and to discuss its roles in the development of the gastric cancer.Methods RT-PCR and real time-PCR were used to detect the expression levels of IL-32 mRNA in the human gastric cancer cell lines（MKN-45 and KATO） and the normal gastric epithelial cell GES-1.Western blot was used to detect the expression levels of protein of IL-32.Results Levels of IL-32 mRNA in the human gastric cancer cell lines were significantly higher than that in the normal gastric epithelial cell.The gray-scale ratio（target genes /internal control）were： GES-1（0.34±0.09）,MKN-45（0.79±0.11）and KATO（0.90±0.17）（P0.05）.The Ct values of real-time PCR showed that the expression level of IL-32 mRNA in MKN-45 was（5.34±1.09）-fold higher than that in GES-1,and KATO were（4.07±1.69）-fold higher.The gray-scale ratio of Western blot were： GES-1（0.30±0.10）,MKN-45（0.92±0.32）and KATO（0.86±0.15）.It showed that IL-32 protein levels in the human gastric cancer cell lines were significantly higher than that in the normal gastric epithelial cell（P0.05）.Conclusion The results showed that the expression of IL-32 in human gastric cancer cell lines were significantly higher than that in the normal gastric epithelial cell.It indicated that the high expression of IL-32 was closely related with progress of human gastric cancer.