鱼卵小分子多肽对SD大鼠骨髓间充质干细胞增殖作用的影响

目的观察不同浓度的鱼卵小分子多肽对SD大鼠骨髓间充质干细胞（BM-MSCs）增殖作用的影响。方法分离、培养SD大鼠BM-MSCs,并采用流式细胞仪进行鉴定。选取第3代细胞进行观察和检测。实验组选取25、50、75、100、125、150、200、250、300μg/ml 9个多肽浓度,对照组加入不含小分子多肽的DMEM完全培养基。各组孵育24 h后采用MTT法检测其细胞增殖情况,确定其最佳作用剂量。在最佳作用量下,于12、16、20、24、32、40、48 h 7个时间点观察鱼卵小分子多肽对SD大鼠BM-MSCs增殖的情况。并采用流式分析鱼卵小分子多肽对BM-MSCs细胞周期的影响。结果鱼卵小分子多肽对BM-MSCs的促增殖作用的最佳浓度为100μg/ml,与对照组相比差异具有统计学意义（P〈0.05）。流式分析显示鱼卵小分子多肽可提高SD大鼠BM-MSCs非G1/G0期细胞比例。结论鱼卵小分子多肽对SD大鼠BM-MSCs有促增殖作用,最佳作用浓度为100μg/ml。

Effects of small molecule polypeptide of roe on the proliferation of rat bone marrow mesenchymal stem cells

Objective To investigate the effects of different concentrations of small molecule polypeptide of roe on the proliferation of rat bone marrow mesenchymal stem cells（BM-MSCs）.Methods Mesenehymal stem cells was separated and cultivated by using explants-attached method and identified by flow cytometry.The third passage of rat BM-MSCs was used in this study.Cells in the experimental group were treated with various concentrations of small molecule polypeptide of roe（25 ～ 400 μg/ml）.After 24 hours of incubation,the effect of small molecule polypeptide of roe on the proliferation of rat BM-MSCs was assayed by MTT method.We also analyzed the effects of small molecule polypeptide of roe on cell cycle progression of the rat BM-MSCs.The experiment was repeated three times.Results Small molecule polypeptide of roe dose-and time-dependently promoted the cell proliferation.Conclusion Small molecule polypeptide of roe can promote the proliferation of rat BM-MSCs.The maximum dose of extract protein of roe was 100 μg/ml.