Stability of a trivalent recombinant protein vaccine formulation against botulinum neurotoxin during storage in aqueous solution |
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Authors: | Vessely Christina Estey Tia Randolph Theodore W Henderson Ian Cooper Julianne Nayar Rajiv Braun Latoya Jones Carpenter John F |
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Affiliation: | Department of Pharmaceutical Sciences. School of Pharmacy, SOP-215, Campus Box C238, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. |
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Abstract: | The adsorption of recombinant botulinum neurotoxin (BoNT) protein-derived vaccine antigens to aluminum salt adjuvants has been previously studied for the development of a trivalent vaccine against the neurotoxins (Vessely et al., in press, J Pharm Sci). The current paper describes an investigation of the stability of recombinant BoNT antigens adsorbed to aluminum salt adjuvants during storage in aqueous solution. Both chemical and physical changes occurred during storage. Phosphate groups in the buffer exchanged with hydroxyl groups on the adjuvant surface. The resulting changes in solution pH and adjuvant surface chemistry promoted more favorable electrostatic interaction between the BoNT proteins and the surface, possibly increasing the affinity of the proteins for the surface during storage. Fluorescence and UV spectroscopy suggested changes to protein structure during storage, whereas differential scanning calorimetry showed changes to thermal processes related to protein conformation and/or surface adsorption. The consequence of the chemical and physical changes to the proteins was a decrease in the ability to desorb protein from the adjuvant surface during storage. Overall, the results of this study emphasize the utility of a thorough characterization of the interactions between protein antigens and aluminum salt adjuvants. |
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