四君子汤抗胃癌活性部位配伍作用及其对细胞周期的影响

目的]研究四君子汤抗胃癌活性部位的配伍作用及其对细胞周期的影响.方法]以SGC-7901胃癌细胞为细胞模型,以接种SGC-7901细胞的裸鼠为动物模型,通过体内、体外方法筛选四君子汤抗胃癌活性部位的最佳配伍；流式细胞仪观察四君子汤活性部位配伍对SGC-7901细胞周期的影响.结果]四君子汤挥发油部位、萜类部位、黄酮部位在体内、体外均体现出配伍协同作用,活性部位配伍组对SGC-7901细胞的药物半数抑制浓度(IC50)为1.574mg/ml,其高、低剂量组体内抑瘤率分别为55.48％和44.52％;四君子汤活性部位配伍组处理后G2/M期SGC-7901细胞显著增加,为12.84％.结论]四君子汤挥发油部位、萜类部位、黄酮部位具有协同配伍作用,能将SGC-7901细胞阻滞于G2/M期.

Multi effect of the various components from Sijunzitang and the influence on cell cycle of SGC-7901

Objective]To study the anti-cancer activity of Sijunzitang’s components compatibility,and its influence on cell cycle.Methods]SGC-7901 stomach cancer cell was used as cell model,nude mouse seeded with SGC-7901 cells was used as animal model,and flow cytometer was used to study cell cycles.Results]Essential oil,terpenoid and flavone components from Sijunzitang have comparative anti-tumor activity in vivo and in vitro.The IC50 on SGC-7901 cell was 1.574mg/ml,and anti-tumor ratio of high and low group were 55.48% and 44.52% respectively.SGC-7901 cells on the period of G2/M were significantly increased.Conclusion]Essential oil,terpenoid and flavone components have the ability of compatibility,and it can stop SGC-7901 cells at G2/M period.