北京地区实验动物绿脓杆菌分离株MLVA分型

目的 通过多位点可变数目串联重复序列分析(multiple-locus variable-number tandem-repeat analysis,MLVA)分型方法,研究北京地区实验动物绿脓杆菌分离株基因型和分布情况.方法 选择13个可变数目重复序列(variable-number tandem-repeat,VNTR)位点,对实验动物及设施中检测出的141株绿脓杆菌的基因组DNA进行重复序列扩增,所得指纹图谱使用BioNumerics软件进行聚类分析,绘制系统发育树和最小生成树(minimum spanning tree,MST).结果 所采用的13个VNTR位点能够对全部分离株进行有效分型.141株绿脓杆菌主要被分为了3个基因群,56个基因型.各群所占比例分别为A群82.3％,B群占12.8％,C群占5.0％,辛普森多样性指数为0.763.同一区域内相邻实验动物单位的绿脓杆菌分离株同源关系较远.结论 MLVA方法对绿脓杆菌具有很好的分型能力,能够有效的追踪绿脓杆菌的来源.北京地区实验动物中绿脓杆菌分离株基因型多态性丰富,但无地域性同源关系.

Multiple-locus Variable-number Tandem-repeat Analysis for Genotyping of Pseudomonas aeruginosa in Beijing

Objective To identify genotypes and potential routes of dissemination of P.aeruginosa isolates in Beijing using Multilocus-locus variable-number tandem-repeat(VNTR) analysis(MLVA).Methods One hundred and forty one experimental animals and facilities isolates of P.aeruginosa were detected by selected 13 VNTRs loci.Then we draw the phylogenetic tree and minimum spanning tree(MST) using BioNumerics software according to fingerprint clustering analysis.Results All isolates of P.aeruginosa was divided into three major genetic Clusters,56 genotypes.The proportion of A cluster is 82.3%,B cluster 12.8%,C cluster 5.0%.The Simpson’s index of diversity is 0.763.The isolates have distant homology in the neighboring facilities.Conclusions MLVA displayed excellent discriminatory power for P.aeruginosa.MLVA can effectively track P.aeruginosa source.The P.aeruginosa isolates of experimental animals have rich polymorphism genotypes and no regional homology in Beijing.